视神经损伤后SDF-1及其受体CXCR4在大鼠视网膜中的分布和表达规律  

Distribution and expression profile of SDF-1 and its receptor CXCR4 in rat retinas after optic nerve injury

作　　者：叶茂[1] 李依孺[1] 徐海伟[1] 何建荣[1] 彭艳丽[1] 吴楠[1] 王一[1] 

机构地区：[1]第三军医大学西南医院全军眼科中心,视觉损伤与再生修复重庆市重点实验室,重庆400038

出　　处：《第三军医大学学报》2014年第7期645-649,共5页Journal of Third Military Medical University

基　　金：国家自然科学基金(30901645;81270996)~~

摘　　要：目的观察Long Evans大鼠视神经损伤后不同时间基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)及其受体CXCR4在视网膜中的分布及表达变化。方法将21只成年Long Evans大鼠分为正常组和视神经损伤后1、3、5、7、15 d及30 d组,每组3只,采用免疫荧光染色和免疫印迹法(Western blot)检测各组大鼠视网膜SDF-1、CXCR4的分布及表达变化。结果免疫荧光染色发现视神经钳夹伤大鼠SDF-1主要定位表达于视网膜内、外界膜之间的星形胶质细胞及Müller细胞,并与GFAP共表达,SDF-1阳性细胞数量随视神经损伤时间延长先增多后减少,伤后5 d达高峰。CXCR4主要表达于视网膜神经节细胞层、外核层及内核层;Western blot证实视网膜SDF-1的水平随视神经损伤出现先升高后降低的趋势,与正常组相比,视神经损伤组第1、3、5、7天水平显著升高(P<0.05)。而CXCR4的表达在视神经损伤后各时间点无明显改变(P>0.05)。结论视神经损伤后,视网膜星形胶质细胞及Müller细胞中的SDF-1表达先增强后减弱,且与视神经损伤后胶质活化相关。Objective To observe the distributions factor-1 （SDF-1） and its receptor CXCR4 in the retinas during and expression profiles of stromal cell-derived repair period after partial optic nerve injury in Long Evans rats. Methods A total of 21 adult rats were randomly and equally divided into 7 groups, normal group, the injury group, and the groups in 1, 3, 5, 7, 15 and 30 d after optic nerve crushing. Immunofluores eence assay and Western blotting was applied to detect the location and the quantity of SDF-1 and CXCR4 expression in the retina. Results Immunofluoreseence staining revealed that the expression of SDF-1 was co localized in the activated GFAP-positive astrocytes and MUller cells between the pre-retinal and post-retinal membrane. The amount of SDF-1 positive cells were in a trend of increasing to decreasing with the injury time prolonging, and reached a summit in 5 d after injury. CXCR4 staining was mainly present in the ganglion cell layer, inner nuclear layer and outer nuclear layer of the retina. Western blotting revealed that the expression of SDF-1 protein was increased at the first day and then decreased at the 7th day after injury. The expression of SDF-1 in 1, 3, 5 and 7 d after optic nerve injuring was increased significantly than normal group （P 〈 O. 05 ）. While, the expression of CXCR4 protein had no change after injury （P 〉 0.05 ）. Conclusion The quantity of SDF-1 is increased first and then decreased in the astrocytes and Mtiller cells after optic nerve injuring, which is related to the activation of glial cells after injury.

关 键 词：SDF-1 CXCR4 视网膜 星形胶质细胞 MULLER细胞 视神经损伤 

分 类 号：R339.146[医药卫生—人体生理学] R341[医药卫生—基础医学]