来氟米特对胶原诱导性关节炎大鼠关节滑膜IL-17和RANKL表达的影响  

Influence of leflunomide on expressions of IL-17 and RANKL in synovium of CIA rats

作　　者：施青[1] 王美美[1] 

机构地区：[1]东南大学附属中大医院风湿免疫科,江苏省210009

出　　处：《江苏医药》2014年第5期515-518,F0003,共5页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK2009276)

摘　　要：目的探讨来氟米特(LEF)对滑膜病变的治疗作用。方法 40只雌性SD大鼠用完全弗氏佐剂乳化的鸡Ⅱ型胶原构建胶原诱导性关节炎(CIA)大鼠关节模型后均分为模型对照(B)组和LEF处理组(A组,LEF 1mg/d灌胃,连续3周);另取10只SD大鼠作为正常对照(C)组。进行关节炎指数(AI)评分,ELISA法检测关节浸液IL-17和细胞核因子κB受体活化因子配基(RANKL)浓度,计算膝关节滑膜病理积分,免疫组化法半定量检测滑膜IL-17和RANKL蛋白表达。结果 A组于给药2周后的AI值低于B组(6.60±1.65vs.9.90±2.38)(P<0.01)。与C组比较,A、B关节浸液IL-17和RANKL浓度、滑膜病理积分、滑膜IL-17和RANKL蛋白表达均增加(P<0.05、P<0.01),但A组上述指标均低于B组(P<0.01)。结论 LEF可能通过抑制IL-17和RANKL在CIA大鼠关节滑膜的表达延缓关节滑膜病变的进展。Objective To study the effects of leflunomide （LEF）intervention on synovial diseases. Methods The collagen-induced arthritis（CIA） models were induced in 40 female SD rats by injecting type Ⅱ collagen and complete Freud＇s adjuvant,which were equally assigned into two groups of A（treated with LEF 1 mg/d gastric gavage for 2 weeks） and B（normal saline control）. Another 10 SD rats were taken as blank controls（group C）. Arthritis was evaluated by arthritis index（AI） and synovial pathological score. The expressions of IL-17 and receptor activator for nuclear factor κB ligand（RANKL） in synovial fluid and synovium were detected by ELISA and immunohistochemistry. Results The AI after treatment was lower in group A than that in group B（6. 60±1.65 vs. 9.90±2. 38）（P〈0. 01）. Compared to group C, the expressions of IL-17 and RANKL in synovial fluid and synovium in groups of A and B were higher（P〈0. 05 or P〈0. 01） ,which were lower in group A than those in group B（P〈0. 01）. Conclusion LEF may attenuate the progress of synovial lesions by inhibiting the exoressions of IL-17 and RANKL in svnovium of CIA rats.

关 键 词：胶原诱导性关节炎 来氟米特 

分 类 号：R593[医药卫生—内科学]