重组亚单位和合成肽防龋疫苗的免疫效能  

Comparison of Immune Effect between the Recombinant Subunit rPAc Anti-caries Vaccine and Synthetic Peptides Vaccine

作　　者：曹茜茜[1] 樊牮 孙晶晶[1] 雷凤翔 陈潇婕 张翕[1] 李字红[1] 樊明文[1] 

机构地区：[1]口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室,武汉大学口腔医学院,湖北武汉430079

出　　处：《口腔医学研究》2014年第3期193-196,200,共5页Journal of Oral Science Research

基　　金：国家自然科学基金项目(编号:81271129)

摘　　要：目的:比较重组亚单位rPAc防龋疫苗和合成肽疫苗诱导小鼠特异性体液免疫和黏膜免疫水平。方法:以rPAc疫苗和合成肽疫苗分别滴鼻免疫小鼠,2、4周后加强免疫,分别收集0、2、4、6、8、10、12周的血清和唾液,用酶联免疫吸附实验(ELISA)检测血清和唾液中特异性抗体的水平。结果:以rPAc防龋疫苗免疫小鼠可产生较合成肽疫苗免疫小鼠更显著的黏膜免疫反应,两组比较差异有显著性(P<0.05)。结论:重组亚单位rPAc防龋疫苗相对于合成肽疫苗具有更明显的黏膜免疫效果。Objective： To compare the efficacy of anti--caries rPAc recombinant subunit vaccine and the synthetic peptides vaccine for inducing protective mucosal immune response in mice. Methods： rPAc and synthetic peptides were respectively delivered into mice by intranasal rout. t 0w, 2w, 4w, 6w, 8w, 10w, 12w after the initial immuni- zation, we collected the serum and saliva of mice, and observed the levels of specific antibodies in serum and saliva by enzyme--linked immunosobent assay（ELISA）. Results： The level of serum specific anti--Pac IgG and salivary anti--PAc IgA in rPAc protein group were significantly higher than synthetic peptides groups（P〈0.05）. Conclu- sion： The anti--caries rPAc recombinant subunit vaccine was sufficiently immunogenic to induce a protective muco- sal immune response in this experimental mice model for dentai caries compared with the synthetic peptides vaccine.

关 键 词：龋齿 重组亚单位疫苗 合成肽疫苗 黏膜免疫 

分 类 号：R392[医药卫生—免疫学]