大肠杆菌密度感应缺陷株中LuxS的表达及其对生物膜形成的影响  

Influence on Biofilm Formation by Expressing LuxS in the E.coli Quorum Sensing Defective Strain

作　　者：王倩[1] 白桦[1] 黄正蔚[2] 

机构地区：[1]大连医科大学口腔医学院口腔内科教研室,辽宁大连116044 [2]上海交通大学医学院附属第九人民医院牙体牙髓病科.上海市口腔医学重点实验室

出　　处：《口腔医学研究》2014年第3期212-215,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(81070826/30872886);上海市科委启明星跟踪科研计划(12QH1401400);上海交通大学医工交叉研究项目(YG2011MS67)

摘　　要：目的：研究在大肠杆菌密度感应缺陷株中表达LuxS蛋白对于生物膜形成相关基因的影响。方法：在大肠杆菌密度感应缺陷株MDA12中表达LuxS蛋白，继而通过Real-time PCR检测菌株间生物膜形成相关基因的表达差异。结果：野生株中生物膜形成相关基因motA、motB表达量约为MDA12的2～5倍，而表达了LuxS蛋白的MDA12中该基因的表达量则为6-8倍，且差异具有统计学意义。结论：大肠杆菌密度感应缺陷株内表达LuxS，可以恢复其密度感应系统继而恢复生物膜形成相关基因的表达。Objective： To investigate the effection of expressing LuxS in E. coli quorum sensing defective strain on its biofilm--related genes. Methods： The LuxS protein was expressed in the E. coli quorum sensing defective strain MDAI2. The biofilm--related genes were investigated by real--time PCR. Results： Compared to MDAI2, the bio- film--related genes motA and motB expressed about 2--fold to 5--fold in wild--type strain. When expressed LuxS in MDAI2, these genes expressed more than the defective strain about 6--fold to 8--fold, and the differences were statistically significant. Conclusion： The biofilm--related genes could be recovered by expressing the LuxS protein in quorum sensing defective strain of E. coli.

关 键 词：密度感应 生物膜 LuxS蛋白 AI-2 

分 类 号：R378[医药卫生—病原生物学]