超声造影评价重组人血管内皮抑制素对兔腹主动脉粥样斑块内新生血管的影响  

Evaluation of the effect of endostar on neovascularization within rabbits of abdominal aortic atherosclerotic plaque using contrast-enhanced ultrasonography

作　　者：胡宪八[1] 张超[1] 黄品同[1] 张莹[1] 葛丹[1] 王尧[1] 孔凡雷[3] 翁慧芳[1] 张建民[2] 

机构地区：[1]浙江大学医学院附属第二医院超声科,杭州310009 [2]浙江大学医学院附属第二医院神经外科,杭州310009 [3]杭州市第一人民医院超声科

出　　处：《中华超声影像学杂志》2014年第3期254-257,共4页Chinese Journal of Ultrasonography

基　　金：浙江省科学技术厅科技计划项目资助（2008C33012）;浙江省自然科学基金（Y2080718）;国家自然科学基金面上项目（81071164,81271584）;浙江省卫生厅省部共建计划（WKJ2011-2-007）

摘　　要：目的运用超声造影评价重组人内皮抑制素（恩度）对兔腹主动脉粥样斑块内新生血管的影响。方法对经球囊拉伤腹主动脉后予高脂喂养12周的26只造模成功雄性新西兰大白兔进行常规超声及超声造影检查，检查完毕随机处死8只兔（A组，病理对照组）取腹主动脉斑块标本进行病理检查，剩余被随机分为2组：B组（9只，对照组），再以普通饲料喂养4周；C组（9只，治疗组），再以普通饲料喂养加恩度治疗4周。16周再行常规超声及超声造影检查，检查完毕后立即处死所有模型，作病理学检查。结果12周时各组斑块平均厚度、面积狭窄率、达峰时间（TTP）、峰值强度（PI）、增强强度（EI）之间比较差异均无统计学意义（P〉0．05）。经过4周恩度治疗，C组斑块EI、微血管密度（MVD）及易损斑块比例与B组同期及A组比较均有明显下降，差异有统计学意义（P〈0．05），余各指标与A组比较差异均无统计学意义（P〉0．05）；而B组16周时各指标与A组比较差异均无统计学意义（P〉0．05）。结论恩度短期治疗能抑制斑块内新生血管的生长，有助于斑块的稳定；超声造影能有效地评价恩度对斑块内新生血管的抑制作用。Objective To evaluate the effect of endostar on neovascularization within rabbits of abdominal aortic atherosclerotic plaques using contrast-enhanced ultrasonography （CEUS）. Methods Twenty six male New Zealand rabbits, damaged abdominal aorta by expanded balloon,high-fat feeding for consecutive twelve weeks, made plaque model successfully, were detected using conventional ultrasound examination and CEUS, Group A （eight rabbits） as pathology control group was killed immediately randomly after examination, then every abdominal aortic plaque were taken out for pathological examination and immunohistochemical detection. Survivals were divided into two groups randomly：Group B （ nine as control group） fed common diet for another four weeks; Group C （nine as treatment group） injected endostar besides common diet. All survivals were detected using conventional ultrasound examination and CEUS once more in sixteen weeks, then killed immediately and all plaques were made for pathological examination. Results The value of maximum thickness, luminal stenosis rate, time to peak （TTP）, peak intensity（PI） and enhanced intensity （EI） compared among three groups had no significant difference（ P 0.05）. After four weeks treatment, EI, microvessel density （MVD） and the rate of vulnerable plaques in group C were significantly less than group A and the same time group B,and the value of contrast between the two groups was significantly different （ P 〈 0.05）. Others value compared with group A had no significant difference （P 〉 0. 05）. However, every value in group B compared with group A had no significant difference （ P 〉 0.05）. Conclusions El could accurately evaluate the effect of endostar on neovascularization within rabbits of abdominal aortic atherosclerotic plaques, at the same time showed endostar could suppress neovascularization of atherosclerotic plaques, which contributed to them become stable.

关 键 词：超声检查 微气泡 主动脉 腹 斑块 动脉粥样硬化 内皮抑素类 

分 类 号：R445.1[医药卫生—影像医学与核医学] R543.5[医药卫生—诊断学]