饲料中铜离子对斑节对虾血细胞组成、活性和免疫功能的影响  

Effects of dietary copper on subpopulation composition, viability and immune functions of haemocytes in black tigershrimp(Penaeus monodon)

作　　者：冼健安[1] 王安利[1] 

机构地区：[1]华南师范大学生命科学学院,广东省水产健康安全养殖重点实验室,生态与环境科学广东普通高校重点实验室,广东广州510631

出　　处：《海洋科学》2013年第12期40-46,共7页Marine Sciences

基　　金：国家自然科学基金资助项目(30671628和31302164);广东省自然科学基金重点项目(S2011020003256和S2012040008093);中国博士后科学基金资助项目(2012M511829);广东省教育部产学研结合资助项目(2011B090500009);广东省科技计划项目(2012B02030-7004);广东省重点实验室建设项目(2009A060800019)

摘　　要：以不同铜离子添加量（0,10,25,40,55和110 mg/kg）的饲料喂养斑节对虾（Penaeus monodon）8周,测定对虾的血细胞总数（THC）,并应用流式细胞术测定血细胞中含颗粒细胞（小颗粒细胞和大颗粒细胞）的比例、血细胞凋亡率、活性氧（ROS）含量和酯酶活性。结果显示,当铜添加量为10-55 mg/kg时,与对照组相比,对虾的 THC、血细胞凋亡率、ROS 含量和酯酶活性均没有显著的变化（P〉0.05）；铜添加量为25-110 mg/kg时,含颗粒细胞的比例显著提高（P〈0.05）；铜添加量为110 mg/kg时, ROS含量显著高于其他组（P〈0.05）,血细胞凋亡率高于25和55 mg/kg组（P〈0.05）, THC低于55 mg/kg组（P〈0.05）,酯酶活性低于25mg/kg组（P〈0.05）。这些结果表明,适宜的饲料铜含量有利于两类颗粒细胞的生成；铜添加过量（110 mg/kg）会诱导血细胞ROS的产生,造成氧化损伤,从而导致凋亡率上升和THC下降。综上所述,在本实验日粮条件下,针对斑节对虾的血细胞功能,其饲料中铜适宜添加含量范围为25-55mg/kg。The black tigershrimps （Penaeus monodon） were fed with diets supplemented copper at different con-centrations （0, 10, 25, 40, 55 and 110 mg/kg） for 8-week, and the total haemocyte count （THC）, percentage of cells contained granular, apoptotic cell ratio, reactive oxygen species （ROS） production and esterase activity were ana-lyzed. The results showed that THC, apoptotic cell ratio, ROS production and esterase activity were not affected by 10-55 mg/kg supplemental Cu, compared to the control group. Percentage of cells contained granular was signifi-cantly increased when the shrimpswere fed with diets containing 10-55 mg/kg supplemental Cu. In shrimps fed with diets containing 110 mg/kg supplemental Cu, the ROS production in haemocytes was significantly increased, and the apoptotic cell ratio was higher than that of the shrimps fed with diets supplemented with 25 and 55 mg/kg Cu, while THC was lower than that of the shrimps fed with diets supplemented with 55 mg/kg Cu, and the esterase activity was lower than that of the shrimps fed with diets supplemented with 25 mg/kg Cu. These results indicate that adequatedietary Cu concentration could increase the number of cells contained granular. Excess supplemental Cu （110 mg/kg） will induce the production of ROS in haemocytes, and then induce apoptosis and THC decreaseby oxidative damage.In conclusion, the adequate supplemental Cu concentration in diet for haemocyte functions in P. monodon is 25-55 mg/kg.

关 键 词：铜 斑节对虾(Penaeus monodon) 血细胞 活性 免疫 

分 类 号：S963.7[农业科学—水产养殖]