青海省胡卢巴根瘤菌的抗逆性  

Stress Resisitance of Rhizobia in Trigonella foenum-grecum L. in Qinghai Province

作　　者：高晓杰[1,2] 刁治民[2] 范建平[1,2] 高宁[1] 侯颖春[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062 [2]青海师范大学生命与地理科学学院,西宁810008

出　　处：《湖北农业科学》2013年第24期6020-6022,共3页Hubei Agricultural Sciences

摘　　要：从青海省的西宁、大通和乐都各城区采集豆科植物胡卢巴（Trigonella foenum-grecum L.）的根瘤,经分离、纯化、镜检、回接获得10株未知菌株,对获得的根瘤菌进行耐盐、耐酸碱、抗生素抗性和温度敏感性等抗逆性测定.结果表明,供试菌株对酸碱的耐性比较强,所有菌株都能在pH 5～9的范围内生长;供试菌株耐盐性较强,有4株菌株能在40 g/L NaCl上生长,其中l株菌株能在50 g/L NaCl上生长;对温度的适应范围较宽：各菌株对不同种类、不同浓度抗生素的抗性存在差异.筛选出3株耐盐、耐酸碱、生长温度范围广和抗生素抗性较强的菌株,分别为QXT805、QXT808、QXT809.Several biochemical characteristics of 10 rhizobia in Trigonella foenum-grecum L.including resistance to salt,acid-alkali,temperature variation from Xining,Datong and Ledu from Qinghai province were studied.The results showed that all strains tested had good tolerance to acid-alkali which could grow under pH 5～9.The strains tested had good tolerance to salt.4 strains could grow under 40 g/L NaCl and 1 strain couldgrow under 50 g/L NaCl.The toleration range of the strains to temperature was wide.Resistance of different strain types and different concentration of the antibiotics were different.3 strains including QXT805,QXT808 and QXT809 having high tolerance to salt,acid-alkali,temperature variation and intrinsic antibiotics were selected.

关 键 词：根瘤菌 抗逆性 胡卢巴(Trigonella foenum-grecum L ) 青海省 

分 类 号：Q939.114[生物学—微生物学] S542[农业科学—作物学]