反相高效液相色谱法对人血浆中甲氨蝶呤的测定  

Determination of Methotrexate in Human Plasma with RP-HPLC

作　　者：廖建华[1] 余磊[1] 李银保[1] 李青松[2] 张赛男[1] 

机构地区：[1]赣南医学院药学院,江西赣州341000 [2]赣南医学院科研中心,江西赣州341000

出　　处：《湖北农业科学》2013年第24期6145-6147,共3页Hubei Agricultural Sciences

基　　金：江西省自然科学基金项目(2010GZY0023)

摘　　要：建立反相高效液相色谱法测定甲氨蝶呤血药浓度的方法,以指导临床合理用药.采用Agilent ZOBAX SB-C18柱（150 mm×4.6 mm,5μm）;流动相为0.025 mol/L磷酸二氢钠溶液（pH 6.30）-甲醇（76/24,V/V）;流速为1.00 mL/min;检测波长为313 nm;柱温为35℃;1O％（V/V）高氯酸为蛋白沉淀剂.结果表明,血浆中甲氨蝶呤浓度在0.05～5.00 μg/mL（Y1=35.556 C＋0.771,r=0.999 9）和5.00～100.00μg/mL（Y2=36.727C ＋1.700,r=0.9999）范围内与峰面积呈良好线性关系,平均回收率为99.03％和97.04％.日内精密度小于1％,日间精密度小于2.5％（n=6）,定量浓度为0.05 μg/mL.该方法色谱条件稳定、操作简单、出峰时间快、线性范围宽、精密、准确、特异性强、回收率高,适用于甲氨蝶呤血药浓度的监测.A RP-HPLC method for detecting methotrexate （MTX） in human plasma was established.The sample was separated by Agilent ZOBAX SB-C18 （150 mm×4.6 mm,5 μm） column with mobile phase of sodium acetate buffer （pH 6.30）-methanol （76/24,V/V） at a flow rate of 1.0 mL/min.The wavelength of detection was 313 nm and the column temperature was 35 ℃.The plasma sample was treated with 10％ perchloric acid for sedimentation.The results showed that the good linearity within 0.05～5.00 μg/mL（Y1=35.556 C＋0.771,r=0.999 9）and 5.00～100.00 μg/mL（Y2 =36.727 C＋1.700,r =0.999 9）were observed.The average recovery rates were 99.03％ and 97.04％,respectively.The RSD with intra-day was less than 1％ and the inter-day was less than 2.5％（n=6）.The LOQ was 0.05 μμg/mL.The established method was simple,accurate,sensitive and reproducible with large-scale of detection range,suitable for monitoring the blood concentration of methotrexate.

关 键 词：反相高效液相色谱法 甲氨蝶呤 血药浓度 

分 类 号：R927.2[医药卫生—药学]