体外诱导神经元样细胞对大鼠脑损伤模型修复的影响  

Repairment of In Vitro Induced Neuron-like Cells on the Brain Injury in Rats

作　　者：魏会平[1] 朱登祥[1] 张爱兰[1] 宋小青[1] 崔乐[2] 

机构地区：[1]河北北方学院基础医学院生物教研室 [2]河北北方学院

出　　处：《神经药理学报》2013年第6期22-28,共7页Acta Neuropharmacologica

基　　金：国家自然科学基金项目(No.81274005);河北省张家口市科技局发展项目(No.0911021D-4)

摘　　要：目的:探讨大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)诱导为神经元样细胞后对大鼠脑损伤模型修复的影响。方法:骨髓贴壁法分离培养大鼠BMSCs并采用流式细胞术进行鉴定,川芎嗪诱导其分化,将诱导24 h的细胞直接注射移植到大鼠脑缺血模型海马区组织,检测分化的神经元样细胞在神经系统微环境中存活情况及病理变化。结果:细胞移植组病理变化与脑损伤模型组相比,神经细胞变性、坏死数量明显减少,间质水肿较轻。结论:骨髓间充质干细胞诱导为神经元样细胞后移植入对大鼠脑损伤的修复有一定的作用。Objective：To investigate the effect of neuron-like cells induced from bone marrow mesenchymal stem cells（BMSCs）in the repairment of brain injury in rats. Methods：BMSCs were isolated and cultured from rat’s bone marrow by adherent method,identified by flow cytometry. BMSCs were induced into neuron-like cells using ligustrazine. 24 hours after neuron-like cell induction,the cells were directly transplanted into the hippocampus of rats with brain injury,and the survival of neuron-like cells in microenvironment of nervous system and pathological changes was examined. Results：Compared to rats with brain injury alone,rats that received neuron-like cells transplantation showed a decreased number of nerve cell degeneration and necrosis and less significant interstitial edema. Conclusion：Neuron-like cells induced from BMSC can improve brain function of rats with brain injury.

关 键 词：脑缺血模型 骨髓间充质干细胞 神经元样细胞 

分 类 号：Q254[生物学—细胞生物学]