血清嗜酸粒细胞阳离子蛋白、IgE和外周血嗜酸粒细胞与儿童支气管哮喘关系的研究  

A relative research between serum ECP, IgE, peripheral blood eosinophils and bronchial asthma in children

作　　者：王化凤 伦立民[1] 边立忠[3] 

机构地区：[1]青岛大学医学院附属医院检验科,山东省在职硕士研究生266101 [2]淄博市张店区人民医院 [3]淄博市齐都医院科教科

出　　处：《中华临床医师杂志（电子版）》2013年第23期81-83,共3页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的探讨儿童支气管哮喘严重程度与血清嗜酸粒细胞阳离子蛋白(eosinophil cationic protein,ECP)、总IgE(Total IgE,TIgE)、嗜酸粒细胞(eosinophils,EOS)水平的关系;了解儿童支气管哮喘常见过敏原,为儿童支气管哮喘的预防、病情判断及治疗提供依据。方法据儿童哮喘严重程度分级标准将95例哮喘患儿分为间歇发作组(n=23)、轻度组(n=29)、中度组(n=23)、重度组(n=20)四个组,选取20例健康体检儿作为对照组。采用ImmunoCAP100E体外过敏原检测系统定量测定血清ECP、TIgE、过敏原特异性IgE(Specific IgE,SIgE)的水平,Sysmex XT-2000i血球分析仪进行EOS计数。结果 95例患儿吸入性过敏原SIgE阳性率为71.58%,以户尘螨、粉尘螨、屋尘为主,分别占69.47%、68.42%、32.63%;食入性过敏原SIgE阳性率为22.11%,以虾、螃蟹为主,分别占21.05%、18.95%。血清ECP在哮喘各组与对照组;轻、中、重三组间均有显著性差异,P均<0.05,最高值出现在重度组。血清TIgE在轻度组、间歇发作组、对照组三组之间差异均无统计学意义,P均>0.05。EOS在哮喘各组与对照组间均有显著性差异,P均<0.05;在重度与中度,中度与轻度,轻度与间歇发作组间差异均无统计学意义,P均>0.05。血清ECP水平与TIgE、EOS水平呈正相关(r=0.615,P<0.01;r=0.533,P<0.01);TIgE水平与EOS水平呈正相关(r=0.239,P<0.05)。结论血清ECP水平更能准确地反映气道炎症的程度,较TIgE和EOS更为敏感,可作为临床检测气道炎症的客观指标。户尘螨、粉尘螨和屋尘是导致3岁以上儿童支气管哮喘病情发作的主要过敏原。Objective To investigate relationship between serum ECP, Total IgE, specific IgE, eosinophils counts and the severity of bronchial asthma in children; To understand the allergens categories of children with bronchial asthma and provide scientific basis for its prevention, the condition judgment and therapy. Methods According to the children＆#39;s asthma severity classification standards, 95 asthma cases were divided into intermittent episodes of group （n=23）, mild group （n=29）, moderate group （n=23）, severe group （n=20）. 20 healthy children selected as a control group. The serum levels of ECP, TIgE and SIgE were quantitatively measured by ImmunoCAP 100E automated system （Phadia AB, Sweden）. EOS counts were detected by Sysmex XT-2000i Hematology Analyzer. Results In 95 cases of children, inhalant allergens sIgE positive rate was 71.58% which house dust mites, dust mites and house dust mainly accounted for 69.47%, 68.42% and 32.63%, respectively. Ingested allergens sIgE positive rate was 22.11% which shrimp and crab mainly accounted for 21.05% and 18.95% respectively. Serum ECP in between asthma each group and the control group, in mild, moderate and severe groups among the three groups were significantly different, P＆lt;0.05, the highest value appears in the severe group. Serum TIgE in mild group, intermittent episodes and control group among the three groups showed no significant difference, P＆gt;0.05. Peripheral blood eosinophils in between asthma each group and the control group were statistically significant differences P＆lt;0.05. between severe and moderate, moderate and mild, mild and intermittent group were not statistically significant, P＆gt;0.05. The serum ECP level was positive correlation with TIgE and EOS counts（r=0.615, P＆lt;0.01;r=0.533, P＆lt;0.01）. The serum TIgE level was positive correlation with EOS counts（r=0.239, P＆lt;0.05）. Conclusions Serum ECP levels reflect the degree of airway inflammation more accurately which are more sensitive than TIgE and EO

关 键 词：哮喘 嗜酸细胞 嗜酸性粒细胞阳离子蛋白 

分 类 号：R725.6[医药卫生—儿科]