抗原葡萄球菌肠毒素B诱导大鼠肾细胞移植免疫耐受形成过程中对CD4+CD25+T细胞的影响  

To explore the effect of CD4+CD25+ T cell in ectogenic kidney cells-transplanted rats with immunotolerance induced by staphylococcal entemtoxin B

作　　者：周力[1] 张川[1,2] 

机构地区：[1]哈尔滨医科大学附属第二医院泌尿外科,150086 [2]成都市第五人民医院泌尿外科

出　　处：《中华临床医师杂志（电子版）》2013年第23期199-201,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：黑龙江省卫生厅(中医管理局)科技计划攻关项目(GC06C40804)

摘　　要：目的探讨超抗原葡萄球菌肠毒素B(SEB)诱导大鼠肾细胞移植免疫耐受形成过程中对CD4+CD25+T细胞的影响情况。方法 10只LEW大鼠作为供体,20只F344大鼠作为受体,设为对照组和SEB组各10只。取供体鼠肾脏细胞注入受体鼠血中建立鼠肾细胞移植模型。SEB组大鼠注射剂量为3.0 mg/kg,对照组大鼠注射同量生理盐水。分别用流式细胞仪法和放射免疫法测定受体鼠血中在移植前后第5、10、20天CD4+CD8+T细胞数量百分数和IL-2、TGF-β含量,对结果进行比较和统计学分析。结果肾移植模型成功率100%,SEB组大鼠外周血中CD4+CD25+T较对照组第5天和第10天明显增高(P<0.05),但第20天无显著差异。对照组血中IL-2不断增高,在第10天、20天时明显高于SEB组;TGF-β表达量无论对照组及SEB组均逐步增加,但SEB组较对照同期增加显著(P<0.05)。结论 SEB通过影响CD4+CD25+T的表达,调节T细胞亚群的分布,对免疫排斥产生调节作用,在诱导免疫耐受中具有一定的作用。Objective To explore the effect of CD4＋CD25＋T cell in ectogenic kidney cells-transplanted rats with immunotolerance induced by SEB （staphylococcal entemtoxin B）. Methods 10 LEW rats as donors, 20 F344 rats as receptors, set as control group and SEB group 10 each. Take donor rat kidney cells injected into receptor in rat blood rat renal cell transplantation model was established. SEB group rats injected dose of 3.0 mg/kg, and the control group rats the same amount of saline injections. Cells respectively with the method and avoid method determination of receptors in rat blood transplantation before and after five days, ten days, 20 days of CD4＋CD8＋ T cell count percentage and IL-2, TGF-beta content, comparison and statistical analysis of the results. Results Renal transplantation model success rate is 100%; CD4＋CD25＋T cell of peripheral blood in SEB group significantly increased than the control group at 5 and 10 days （P＆lt;0.05）, but at 20 days there was no significant difference. Control group in increasing serum IL-2 at 10 and 20 days are significantly higher than SEB group; TGF-beta expression quantity regardless of the control group and SEB group gradually increased, but the SEB increased significantly than control group over the same period （P＆lt;0.05）. Conclusions SEB influencing the expression of CD4＋CD25＋ T to adjust the distribution of T cell subgroup, produces regulation to immune rejection, which have a certain role in the induction of immune tolerance.

关 键 词：肾移植 移植耐受 CD4 CD25 CD4 CD25 

分 类 号：R692.1[医药卫生—泌尿科学]