PPAR-γ激动剂15d-PGJ2对兔耳增生性瘢痕的影响  

Effect of peroxisome proliferator-activated receptor-γ activator 15d-PGJ2 on hypertrophic scar in rabbit ear

作　　者：潘姝[1] 赵孝开 谢有富[1] 方力[2] 戴丽冰[3] 

机构地区：[1]暨南大学医学院第四附属医院广州市红十字会医院烧伤整形科,广州510220 [2]暨南大学医学院第四附属医院广州市红十字会医院病理科,广州510220 [3]暨南大学医学院第四附属医院广州市红十字会医院创伤外科研究所,广州510220

出　　处：《中华临床医师杂志（电子版）》2013年第23期209-211,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：广州市医药卫生科技重点项目(20121A021010);广东省医学科研课题(A2011482);广州市医药卫生科技一般引导项目(201102A213138).

摘　　要：目的观察过氧化物酶体增殖物激活受体-γ(peroxisome proliferator activated receptor-γ,PPAR-γ)的配体l5-脱氧-△12,14-前列腺素J2(15-deoxy-△12,14-prostagliandxin J2,15d-PGJ2)对兔耳增生性瘢痕CD34及转化生长因子-β1(transforming growth factor-β1,TGF-β1)的影响,探讨15d-PGJ2防治增生性瘢痕的机制和可行性。方法选取新西兰大白兔9只,在兔耳腹侧面制作2 cm×2 cm全层皮肤缺损创面,每耳2个,共计36个,建立兔耳增生性瘢痕动物模型,随机分为实验组和对照组,分别用15d-PGJ2及生理盐水行瘢痕内注射,1次/d,连续注射7 d。停止注射后第3、6、17天取材。应用免疫组织化学方法检测CD34和TGF-β1的表达。结果兔耳创面愈合后均出现不同程度类似人增生性瘢痕组织块,与对照组比较,实验组注射15d-PGJ2后瘢痕逐渐变平变软,颜色变浅,在各时间点CD34及TGF-β1的表达均较对照组低,差异有统计学意义(P<0.05)。结论 PPAR-γ的配体15d-PGJ2可减少瘢痕内CD34、TGF-β1的含量,引起瘢痕萎缩,有一定的防治瘢痕增生的作用,为其治疗增生性瘢痕的临床应用提供了一定的理论依据。Objective To investigate the effect of 15d-PGJ2 on the expression of CD34 and TGF-β1 on hypertrophic scar in rabbit ear and the possibility of hypertrophic scar treated by 15d-PGJ2. Methods 9 New Zealand white rabbits were used to establish a hypertrophic scar model on the rabbit ear.The wounds were established as follows：2 cm＆#215;2 cm wounds with total skin loses on the ventral side, 2 wounds for each ear, totally 36 wounds. The wounds were randomly divided into the 15d-PGJ2 treatment group and normal saline control group. 10μl 15d-PGJ2 or normal saline was injected into the ear scar once a day for 7 days. At 3, 6 and 17 days after the injection, the expression of CD34 and TGF-β1 was detected by immunohistochemical method. Results Excessive dermal scars on rabbit ear that were similar to human hypertrophic scar appeared in the two groups. Compared with the NS-treated scar group, the 15d-PGJ2-treated scars appeared to be smaller, softer, flatter and lighter in color. The expression of CD34 and TGF-β1 in the 15d-PGJ2 group was significantly decreased as compared with that in the control group at different time points（P＆lt;0.05）. Conclusion 15d-PGJ2, the ligand of PPAR-γ, can reduce the expression of CD34 and TGF-β1 of hypertrophic scar in the rabbit ear and plays an important role in the prevention and treatment of hypertrophic scar.

关 键 词：瘢痕 PPARΓ 转化生长因子Β1 CD34 

分 类 号：R965[医药卫生—药理学]