microRNA与冠状动脉粥样硬化性心脏病的相关性研究  

Association between microRNA and coronary artery disease

作　　者：石晓凤[1] 韦小未 朱建兵[1] 徐芒华[1] 张宗棨[1] 张俊峰[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院心内科,201999

出　　处：《中华临床医师杂志（电子版）》2013年第24期39-42,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：上海市自然科学基金面上项目(12ZR1417400);上海市教委优青项目(jdy11001);上海交通大学医学院附属第三人民医院优青项目(11YYQ03)

摘　　要：目的探讨miR-126,miR-130a,miR-222在冠心病患者外周血浆中的表达水平及其临床意义。方法选择冠心病患者79例(45例轻度组、34例重度组),冠状动脉阴性对照组24例,收集两组样本临床资料;运用Real-time PCR法检测样本血浆miR-126、miR-130a、miR-222表达水平;分析其表达差异及与脂蛋白a相关性,评估血浆miR-126、miR-130a、miR-222在冠心病中的表达水平及其临床意义。结果 (1)与对照组相比,冠心病组患者血浆miR-126和miR-222的表达水平降低和升高,分别是对照组的0.08倍和4.23倍,差异有统计学意义(P<0.05);miR-130a表达水平降低,是对照组的0.32倍,但差异无统计学意义(P>0.05)。(2)以改良Gensini评分对冠心病患者进行亚组分析,冠心病轻度组与重度组miR-126的表达水平分别为对照组的0.15倍及0.004倍,miR-222的表达水平分别为对照组的2.77倍及7.38倍,差异均有统计学意义(P<0.05),并且miR-126、miR-222表达水平变化与改良Gensini评分相关。(3)冠心病组中患者Lpa与miR-126、miR-130a、miR-222表达水平均无相关性。结论冠心病患者外周血浆中miR-126、miR-222表达水平较冠状动脉阴性对照组有显著差异,且表达水平变化与冠状动脉狭窄程度密切相关。Objective To investigate the expression and clinical implication of peripheral plasma miR-126, miR-130a, miR-222 in patients with coronary artery disease（CAD）. Methods 79 patients with CAD （CAD group： 45 mild, 34 severe） and 24 controls without CAD were included. General clinical data and related biochemical parameters were collected. Plasma miR-126, miR-130a, miR-222 levels were detected with quantitative real-time polymerase chain reaetion（qRT-PCR） analysis and the expression differences were analyzed. The correlation between miR-126, miR-130a, miR-222 and lipopmtein（a） were analyzed also. Results （1） Compared with control group, the expression level of plasma miR-126 was significantly decreased in CAD group （fold 0.08） and miR-222 was significantly increased in CAD group （fold 4.23）（P〈0.05）; miR-130a was decreased （fold 0.32） （P〈0.05）. （2） In CAD subgroup of improved Gensini method, the expression level of plasma miR-126 and miR-222 were significantly different in mild and severe group, miR-126 （mild with fold 0.15, severe with fold 0.004） （P〈0.05）, miR-222 （mild with fold 2.77, severe with fold 7.23）（P〈0.05）. The expression level changes of plasma miR-126 and miR-222 were closely related to improved Gensini scores. （3）There was no correlation between miR-126, miR-130a, miR-222 and lipoprotein（a） in CAD group （P〉0.05）. Conclusion Compared with control group, the expression levels of peripheral plasma miR-126 and miR-222 were significant difference in patients with CAD. The expression level changes of plasma miR-126 and miR-222 were closely related to the severity of Coronary artery stenosis.

关 键 词：冠心病 微RNAS 聚合酶链反应 

分 类 号：R541.4[医药卫生—心血管疾病]