不同洗脱液对环氧乙烷生物指示剂活菌洗脱效果及抗力测定影响  

IMPACT OF DIFFERENT ELUENT AFFECTING ELUTION OF VIABLE TEST MICROORGANISMS OF THE ETHYLENE OXIDE BIOLOGICAL INDICATORS AND RESISTANCE

作　　者：陈越英[1] 吴晓松[1] 徐燕[1] 陈文森[2] 谈智[1] 

机构地区：[1]江苏省疾病预防控制中心,南京210009 [2]江苏省人民医院

出　　处：《中国消毒学杂志》2014年第3期239-241,共3页Chinese Journal of Disinfection

基　　金：卫生部国家标准(2008-07-01);江苏省"十二.五"科教兴卫工程(ZX201109):"突发公共卫生事件"应急处置创新平台

摘　　要：目的研究不同洗脱液对环氧乙烷生物指示剂的菌量及抗力测定结果的影响。方法通过细菌定量检测方法,比较不同洗脱液对生物指示剂上活菌洗脱效果及其对生物指示剂抗力的影响。结果 6种洗脱液与5种稀释液对同一批次的生物指示剂载体上细菌洗脱后,细菌回收效果最好的是用5 000 mg/L吐温-80的营养肉汤洗脱后,用浓度为5 000 mg/L吐温-80的PBS,回收菌数为7.03×106cfu/片。回收效果最差的是无菌生理盐水,平均回收菌数为3.78×106cfu/片。回收菌数数值越大D值越小,ST值相应变大和KT值变小。结论洗脱效果最好的洗脱液和相应稀释液是吐温-80营养肉汤与吐温-80 PBS,生物指示剂回收菌数越高,其D值更接近真值。Objective To study impact of different eluent affecting elution of viable test microorganisms of the ethylene oxide （EO） biological indicators （BIs） and resistance. Methods Bacterial quantitative detection method was used to compare the impact of different eluent on viable microorganisms of EO BIs and its resistance. Results Nutrient broth con- taining 0. 5% Tween -80 eluted with PBS containing 0. 5% Tween -80 dilution had a best recovery rate among 6 eluents and 5 diluents, which got 7.03 × 106cfu/unit. On the contrary, sterile saline solution had the worst recovery effect, which got 3.78 × 106cfu/unit. The more recovery viable microorganism count is, the lower D value is, which lead to higher ST value and lower KT value. Conclusion The best eluent and diluent is nutrient broth containing 0. 5% Tween - 80 and PBS containing 0. 5% Tween -80. The more recovery viable microorganism of BIs are, the closer D value get to truth value.

关 键 词：洗脱液 生物指示剂 活菌计数 抗力 

分 类 号：R187[医药卫生—流行病学]