Affymetrix基因芯片研究高血压对小鼠血管基因表达的影响  

Microarray analysis on the expression of gene in mice vascular of hypertension

作　　者：王吉静[1,2] 李玉琳[1,2] 王月丽[1,2] 郑帅[1,2] 王绿娅[1,2] 李汇华[1,2] 杜杰[1,2] 

机构地区：[1]首都医科大学附属北京安贞医院 [2]北京市心肺血管疾病研究所省部共建心血管重塑相关疾病教育部重点实验室,北京100029

出　　处：《心肺血管病杂志》2014年第2期258-262,共5页Journal of Cardiovascular and Pulmonary Diseases

基　　金：国家自然科学基金(81230006);科研基地建设;心血管重大疾病防治协同创新中心(PXM2013_014226_000006)

摘　　要：目的:利用基因芯片技术研究高血压早期血管损伤中基因表达变化。方法:30只8周龄雄性C57BL/6J小鼠,分为对照组和Ang II(血管紧张素II)组,采用置入式胶囊渗透压泵分别灌注0.9%氯化钠液和Ang II(1 500 ng·kg-1·min-1),于第3天,第7天取胸主动脉,提取核糖核酸(RNA),进行基因芯片分析;利用鼠尾套法检测Ang II灌泵3 d及7 d后小鼠尾动脉血压、苏木精伊红染色(HE)染色检测血管结构;实时定量聚合酶链式反应(PCR)验证Ang II灌泵7 d时部分芯片结果。结果:与对照组相比,Ang II组在灌泵7 d时血压升高[(104±4)vs.(146±6)mmHg(1mmHg=0.133kPa),P<0.001];基因芯片结果显示3d时有67个基因表达上调,27个基因表达下调,7d时有31个基因表达上调,没有检测下调基因;PCR验证磷酸分泌蛋白1(SPP1),金属蛋白酶组织抑制因子1(Timp1),4域跨膜超家族A,4C(MS4a4c),胰岛素样生长因子结合蛋白2(Igfbp2),重组激活基因1(Rag1)均表达升高。结论:Ang II导致高血压情况下,血管组织基因表达变化,可能参与高血压血管损伤的过程。Objective：To identify the genes of which responsible for hypertension induced vascular injury.Methods：30 C57BL/6J male mice were randomly divided into control group （n =12） and angiotensin Ⅱ group （n =18）,using osmotic mini-pumps were implanted capsules infusion saline and Ang Ⅱ 1 500 ng.kg-1 · min-1 ; Blood pressure was measured by tail-cuff plethysmography method.At day 3 and 7,Ang Ⅱ-or salinetreated mice were euthanized,and the mRNAs were isolated from thoracic aorta and were analyzed by microarray.At day 7,the mRNA expression of SPP1,Timpl,MS4a4c,Igfbp2,Rag1 were analyzed for real-time PCR.Results：Compared with the control group,blood pressure was significantly increased at 7 days after Ang Ⅱ infusion [（104 ± 4） vs.（146 ± 6） mmHg （1mmHg =0.133 kPa）,P ＜ 0.001] ; Microarray analysis showed that 67 genes were upregulated and 27 genes were downregulated in 3 days and there were 31 genes upregulated after Ang Ⅱ infused 7 days; Real-time PCR confirmed that SPP1,Timp1,MS4a4c,Igfbp2,Rag1 expression in aorta after 7 days Ang Ⅱ-infused animals were significantly increased.Conclusion：Ang Ⅱ infusion leads to hypertension and increases many genes expression,these multifunctional genes could be responsible for hypertension-induced vascular injury.

关 键 词：高血压 血管损伤 基因芯片 炎症反应 血管紧张素Ⅱ 

分 类 号：R543[医药卫生—心血管疾病]