红花黄色素对体外循环肺再灌注损伤的保护作用  

The effect of safflor yellow on lung injury with cardiopulmonary bypass in pigs

作　　者：杨璟[1] 臧宝霞[2] 何美玲[1] 刘锋[1] 于洋[3] 刘晓[3] 龚庆成[1] 金鸣[1] 

机构地区：[1]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所体外循环和机械辅助科,北京100029 [2]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所药理研究室,北京100029 [3]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所心脏外科,北京100029

出　　处：《心肺血管病杂志》2014年第2期272-275,共4页Journal of Cardiovascular and Pulmonary Diseases

基　　金：国家自然科学基金(30572344)

摘　　要：目的:探索红花黄色素(SY)对体外循环(cardiopulmonary bypass,CPB)后肺再灌注损伤的保护作用。方法:将24只小型猪随机分为四组,每组6只。假手术组(A组);CPB对照组(B组);红花黄色素0.26 g/kg组(C组);红花黄色素0.37 g/kg组(D组)。CPB前、肺再灌注30、60、120和240min采集相应的动脉血(或肺静脉血),检测丙二醛(MDA)含量、肿瘤坏死因子(TNF-α)、中性粒细胞和单核细胞,再灌注240min后,取右肺下叶相同部位肺组织,测定湿干质量比值。结果:与A组比较,B组的MDA和TNF-α再灌注后显著增高(P<0.05),中性粒细胞和单核细胞也明显升高(P<0.05);与B组比较,C,D组的MDA和TNF-α有所降低,尤其在肺再灌注240min(P<0.01),单核细胞显著降低(P<0.05);D组与B组相比较,中性粒细胞显著降低(P<0.05),湿干重质量比值明显减少(P<0.05);与C组比D组的TNF-α降低明显(P<0.05)。结论:红花黄色素对CPB肺损伤具有缓解作用,高剂量组较低剂量组效果更加明显。Objective：To investigate the effect of safflor yellow on lung injury after cardiopulmonary bypass （CPB） in pigs.Methods：All miniature pigs were randomly divided into 4 groups：sham group （A group）,CPB model group（B group）,safflor yellow 0.26 g / kg group（C group）,safflor yellow 0.37 g / kg group（D group）.Blood samples were collected before CPB and reperfusion 30,60,120 and 240 min.Malondialdehyde （MDA）,tumor necrosis factor （TNF-α）,neutrophils and monocytes were detected.Dry/wet weight ratio of the right lower lobe lung tissue were defined after reperfusion 240min.Results：Compared with A group,MDA and TNF-o in B group were significantly higher after reperfusion （P ＜ 0.05）,neutrophils and monocytes in B group increased significantly as reperfusion （P ＜ 0.05）.Compared with B group,MDA and TNF-α in C,D group were significantly lower （P ＜ 0.05）.With B group,Dry/wet weight ratio in D group was lower （P ＜ 0.05）.Conclusion：Safflor yellow can protect lung injury during cardiopulmonary bypass.

关 键 词：红花黄色素 体外循环 肺再灌注损伤 

分 类 号：R56[医药卫生—呼吸系统]