等离子体-紫外复合诱变选育四羟基环孢菌素衍生物高产菌株  

Screening and Breeding of High Producing Strain for [γ-HyMeLeu^4] CyA by ARTP-UV Composite Mutagenesis

作　　者：章丽[1] 戴梦[1] 郑桂珍[1] 赵颖[1] 刘静[1] 张佳[1] 王富强[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司微生物药物国家工程研究中心,河北石家庄050015

出　　处：《微生物学杂志》2014年第1期68-71,共4页Journal of Microbiology

基　　金：微生物药物技术创新与新药创制产学研联盟(2010ZX09401-403)

摘　　要：筛选可体内转化合成环孢菌素衍生物([γ-HyMeLeu4]CyA)的高产菌株。以稀有放线菌Nonomuraea dietziae为出发菌株,采用常温常压等离子体射流和紫外照射对其进行复合物理诱变。通过该诱变方法获得了具有稳定遗传性能的菌株,其转化效率比出发菌株提高了32%左右。结果表明,等离子体诱变可有效的用于该稀有放线菌,通过等离子体-紫外复合诱变筛选得到的突变菌株可用于工业生产。In vivo transformable [γ-HyMeLeu4] CyA high-producing strain was screened taking rare actinomycetes Nonomuraea dietziae as a starting strain adopting normal temperature and normal pressure ARTP-UV radiation to carry out composite physical mutagenesis. The results showed that a high yielding strain possessing stable genetic performance was obtained using the mutagenesis with transformation efficiency increased by 32% over the starting strain. Therefore, it can be concluded that ARTP mutation can be effectively used for the rare actinomycetes, the mutant strain obtained through the ARTP-UV composite mutagenesis can be used for industrial production.

关 键 词：环孢菌素衍生物 常温常压等离子体 紫外 复合诱变 

分 类 号：Q93[生物学—微生物学]