脱细胞腮腺基质材料制备及生物相容性实验研究  

Study on preparation and biocompatibility of acellular parotid gland stroma

作　　者：甄恩明[1] 姜刚勇[1] 刘洪伟[1] 杨兆安[2] 马建军[1] 

机构地区：[1]济宁医学院附属济宁市第一人民医院,山东济宁272011 [2]温州医科大学附属第一医院,浙江温州325000

出　　处：《济宁医学院学报》2014年第1期20-23,26,共5页Journal of Jining Medical University

基　　金：温州市科技局重大项目科研基金项目(Y20080090)

摘　　要：目的探讨新西兰大白兔脱细胞腮腺基质的生物相容性。方法采用冻融联合酶消化—冷冻干燥法制备的脱细胞腮腺基质材料,通过体外毒性试验、全身急性毒性及亚毒性试验等评价其生物相容性。结果脱细胞腮腺基质材料具有利于腮腺细胞生长的三维网状基质胶原结构。加入浓度为50%、100%的脱细胞腮腺基质浸提液的培养液培养腮腺细胞于3、5、7d时细胞数目与对照组无显著差异(P>0.05)。浸提液注入兔腹腔未引起急性及亚急性毒性反应,心、肝、脾、肺、肾组织学检查未见组织和细胞变性。加入脱细胞腮腺基质浸提液的兔血溶血程度为1.86%。注射浸提液的兔的升高温度的最高值均小于0.6℃,体温升高总和小于1.4℃,符合致热原试验要求。结论脱细胞腮腺基质材料具有很好的生物相容性。Objective To prepare acellular rabbits parotid gland stroma and evaluate its biocompatibility in vitro.Methods The acellular rabbits parotid gland stroma was prepared by the means of enzymatic digestion, freezing and thawing and lyophilization,The biocompatibility of the material was evaluated by acute toxicity experi-ment and cytotoxicity test in vitro.Results Acellular rabbits parotid gland stroma had the characters of possessing the native structure of the parotid gland stroma,which facilitated the growth of the seed cells.Therewas no signifi-cant difference between the control group cells number and the concentration of 50%,100% respectively of acellu-lar rabbits parotid gland stroma leaching liquid nutrient solution culture of parotid gland cells in 3d,5d,7d cells number （P〉0.05）.Leaching liquid injection of rabbit celiac did not induce acute and subacute toxicity,heart,liv-er,spleen,lung,kidney biopsy did not see the tissue and cell degeneration.Hemolysis rate of acellular rabbits parot-id gland stroma was 1.86%.Injection of the leaching solution of rabbit＆#39;s body temperature peak was less than 0.6℃,and the sum of the values of the temperature rise was less than 1 .4 ℃ conformed by the requirement to pyro-gen test.Conclusion These indicated that acellular rabbits parotid gland stroma presented good biocompatibility in vitro,which could be used as a scaffold for tissue engineering artificial salivary gland.

关 键 词：脱细胞腮腺基质 生物相容性 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]