293T细胞对介孔二氧化硅纳米材料的摄取研究  

Experimental Study of Mesoporous Silica Nanomaterials Uptake in 293T Cell

作　　者：田迎[1] 王建东[2] 滕兆刚[1] 卢光明[1] 郑玲[1] 

机构地区：[1]南京军区南京总医院医学影像科,南京210002 [2]南京军区南京总医院医学病理科,南京210002

出　　处：《医疗卫生装备》2014年第3期26-28,共3页Chinese Medical Equipment Journal

摘　　要：目的:研究在脂质体Lipofectamine 2000的介导下,293T细胞对介孔二氧化硅纳米材料(mesoporous silica nanomaterials,MSNs)的摄取变化。方法 :制备和表征MSNs;293T细胞分别与100μg/mL MSNs、MSNs+Lipofectamine2000共孵育,通过透射电镜(transmission electron microscope,TEM)观察及定量分析293T细胞对材料的摄取。结果:TEM、X线衍射及氮气吸附-脱附分析表明,制备的MSNs结构规则、分散性好,尺寸在100 nm左右;TEM定量结果发现,Lipofectamine 2000显著提高了293T细胞对MSNs的摄取,P<0.001。结论:Lipofectamine 2000能提高293T细胞对MSNs的摄取,为MSNs在293T细胞内的生物应用创造了条件。MSNs were synthesized and characterized; 293T cells were incubated with MSNs or MSNs and Lipofectamine 2000 re- spectively, which both had a concentration of 100 μ g/mL. Transmission electron microscope （TEM） was used to analyze the up- take of MSNs in 293T cells. Results The analyses by TEM, X-ray diffraction and Nitrogen absorption-desorption showed the prepared MSNs behaved well in structure and dispersion and had a size of 100 nm. TEM quantitative analysis found that Lipo- fectamine 2000 significantly enhanced the uptake of MSNs in 293T cells, with P〈0.01. Conclusion MSNs uptake promoted by Lipofectamine 2000 lays a foundation for its biological application in 293T cells.

关 键 词：介孔二氧化硅纳米材料 LIPOFECTAMINE 2000 细胞摄取 

分 类 号：R318.08[医药卫生—生物医学工程]