毛细管电泳安培法测定田基黄中的芦丁与槲皮素  

Determination of rutin and quercetin in hypericum japonicum by capillary electrophoresis with amperometric detection

作　　者：邓光辉[1] 王士伟[1] 王辉[1] 屈忠凯[1] 韦福赛 

机构地区：[1]广西民族大学化学化工学院,广西林产化学与工程重点实验室,南宁530006

出　　处：《分析试验室》2014年第4期424-427,共4页Chinese Journal of Analysis Laboratory

基　　金：广西壮族自治区自然科学基金项目(0640038)资助

摘　　要：建立了毛细管电泳电化学分离检测田基黄中生物活性成分芦丁和槲皮素的方法。考察了检测电极电位、缓冲液浓度、pH、运行电压和进样时间对分离的影响。以40cm长，50 μm内径的石英毛细管作为分离通道，运行缓冲液为25mmol／L硼砂（pH9.2）溶液，分离电压12kV，0.3mm直径的铂圆盘电极为检测电极，检测电位1.00V（vs.Ag／AgCl），芦丁和槲皮素在10min内得到良好分离。在上述实验条件下，芦丁和槲皮素分别在8.2×10^-6 ～ 5.2×10^-4 ～ mol／L-与6.8×10^-6 ～ 7.2×10^-4 mol／L范围内与峰面积呈良好线性关系，检出限分别为9.0×10^-7 mol／L（S／N=3）和4.7×10^-7 mol／L（S／N=3）。方法已应用于田基黄药材提取物成分分析。A method based on capillary electrophoresis with amperometric detection was established for the separation and determination of rutin and quercetin in hypericum japonicum. Detection potential, buffer concentration and pH, separation voltage and injection time were investigated to acquire the optimum condition. An uncoated silica capillary column （50 μm i.d. × 40 cm）was used as the separation channel, the two could be well separated analytes within 10 minutes by using 25 mmol/L sodium borate buffer（pH9.2） and 12 kV as the separation voltage. The detection electrode was a platinum disk electrode （0.3mm i. d. ） and the detection potential was 1.00 V （vs. Ag/AgC1）. Under the optimized experimental conditions mentioned above, there was a good linear relationship for rutin ranging from 8.2 × 10^-6 mol/L to 5.2 × 10^-4 mol/L as well as 6.8 × 10^-6 mol/L to 7.2 × 10^-4 mol/L for quercetin. The detection limits for rutin and quercetin were 9.0 × 10^-7 mol/L （S/N = 3） and 4.7 × 10^-7 mol/L （ S/N=3 ）, respectively. The method has been successfully used for the assay of bioactive compounds in hypericum japonicum extracts.

关 键 词：芦丁 槲皮素 毛细管电泳 安培法 

分 类 号：O657.8[理学—分析化学]