盐酸戊乙奎醚结合液体复苏抑制失血性休克大鼠小肠黏膜凋亡的研究  

Study of penehyclidine hydrochloride binding fluid resuscitation on apoptosis of small intestinal mucosa in rats with hemorrhagic shock

作　　者：徐浩锋 张琴[2] 姜久昆[2] 姚峰[2] 

机构地区：[1]浙江省富阳市人民医院药剂科,浙江富阳311400 [2]浙江大学医学院附属第一医院急诊科

出　　处：《中华危重症医学杂志（电子版）》2014年第1期16-19,共4页Chinese Journal of Critical Care Medicine:Electronic Edition

基　　金：浙江省自然科学基金项目(Y2100430)

摘　　要：目的探讨盐酸戊乙奎醚结合液体复苏对失血性休克大鼠小肠黏膜细胞凋亡的影响。方法 32只SD大鼠,制成重度失血性休克模型,分成对照组、假手术组、液体复苏组和盐酸戊乙奎醚结合液体复苏组,每组8只。采用流式细胞仪和FITC-Annexin V/PI荧光染色法检测和比较失血/复苏/急救后各组大鼠小肠黏膜细胞凋亡的发生情况。结果无论采取治疗措施,经历了失血性休克和复苏的大鼠,其小肠黏膜细胞均存在显著的细胞凋亡情况。液体复苏组和盐酸戊乙奎醚结合液体复苏组大鼠的小肠黏膜细胞凋亡率明显高于对照组和假手术组,差异均有统计学意义(P均<0.05)。同时,与液体复苏组相比较,盐酸戊乙奎醚结合液体复苏组大鼠的小肠黏膜细胞凋亡率明显降低,差异有统计学意义(P<0.05)。结论盐酸戊乙奎醚结合液体复苏能有效抑制失血性休克大鼠肠道黏膜细胞的凋亡,从而减少失血/复苏对肠道黏膜完整性的损害,可能有助于改善预后。Objective To investigate the effect of penehyclidine hydrochloride （PHC） binding fluid resuscitation on apoptosis of small intestinal mueosa in rats with hemorrhagic shock. Methods A model of rat with severe hemorrhagic shock and active bleeding was established in 32 SD rats. The rats were randomly divided into the control group, sham group, resuscitation group, and resuscitation ＋ PHC group, with 7 rats in each group. We detected and compared the apoptosis of small intestinal mucosa of survivors after hemorrhage and resuscitation by FITC-Annexin V/PI double staining method and flow cytometry. Results In the early stage of hemorrhagic shock and resuscitation, marked apoptosis occurred in the small intestinal mucosa from the resuscitation and resuscitation ＋ PHC groups. Compared with the control group and sham group, the apoptosis of small intestinal mucosa of resuscitation and resuscitation ＋ PHC groups were obviously increased （all P 〈 0.05）, Simultaneously, the ap0pt0tic rate in the resuscitation group was higher than that in the resuscitation ＋ PHC group （P〈 0.05）. ConclusionsAdministration of penehyclidine hydrochloride （PHC） can obviously reduce the apoptosis of small intestinal mucosa in rats with severe hemorrhagic shock, which may improve the prognosis.

关 键 词：失血性休克 复苏 盐酸戊乙奎醚 小肠 细胞凋亡 

分 类 号：R459.7[医药卫生—急诊医学]