甘草黄酮对2型糖尿病大鼠肝脏中GSK-3β蛋白表达的影响  

Effect of Licorice Flavonoids on GSK-3β Protein Expression in Liver of Type 2 Diabetic Rats

作　　者：金鑫[1] 赵海燕[1] 马永平[1] 

机构地区：[1]北方民族大学生物科学与工程学院,银川750021

出　　处：《天然产物研究与开发》2014年第3期419-422,共4页Natural Product Research and Development

基　　金：国家自然科学基金项目(30960510);北方民族大学2012年研究生创新项目(2012年申报)

摘　　要：本文旨在研究甘草黄酮(LF)对2型糖尿病大鼠肝脏中GSK-3β蛋白表达的影响。采用高脂高糖饲料喂养结合一次性小剂量腹腔注射链脲佐菌素(STZ)的方法建立2型糖尿病(T2DM)实验大鼠模型。将实验大鼠分为正常对照组(CON)、糖尿病模型组(DM)、甘草黄酮治疗组(LF)和阳性对照组(PC)。分别以溶剂(1,2-丙二醇)、甘草黄酮[300 mg/(kg·d)]或吡格列酮[10 mg/(kg·d)]连续灌胃6周。采用蛋白免疫印迹(Westernblotting)方法检测各组大鼠肝脏中GSK-3β的蛋白表达量。研究结果显示,DM组大鼠肝脏组织中GSK-3β的蛋白表达量较CON组显著升高(P<0.01);其在LF组和PC组的表达量与DM组相比均显著降低(P<0.05)。上述结果表明,LF能显著降低2型糖尿病大鼠肝脏中GSK-3β的蛋白表达量,这可能是其改善胰岛素抵抗的分子机制之一。This study aimed to investigate the effect of licorice flavonoids （LF） on GSK-3β protein expression in liver of type 2 diabetic rats. The type 2 diabetic rat model was replicated by high-fat and high-sugar diet combined with low-dose streptozotocin （STZ） injection. The rats were divided into control group （CON）, diabetic model group （DM）, LF treat- ment group （LF） ,and positive control group （PC）. Rats in different groups were administered with solvent （1,2-pro- panediol）,licorice flavonoids [ 300mg/（ kg· d） ] or pioglitazone [ 10mg/（ kg · d） ], respectively. The protein expression level of GSK-3β in liver was detected by Western blotting. The experimental results showed that the protein expression level of GSK-3β in DM group was significantly higher than that of the CON group （P 〈 0. 01 ） ,while its expression level in LF group and PC group significantly reduced （ P 〈 0.05 ） compared with CON group. These results suggested that licorice flavonoids can significantly decrease the protein expression level of GSK-3β in liver of T2DM rats. This might be one of the molecular mechanisms that LF ameliorated insulin resistance.

关 键 词：甘草黄酮 2型糖尿病 胰岛素抵抗 GSK-3Β 蛋白表达量 

分 类 号：R966[医药卫生—药理学]