不同时期添加GTPs对奶牛体外胚胎早期发育的影响  

Effects of Addition of Green Tea Polyphenols on Early Development of Bovine Embryos during In Vitro Fertilization and in vitro Culture

作　　者：傅春泉[1] 李正操[2] 王争光[2] 俞颂东[2] 

机构地区：[1]金华职业技术学院,浙江金华321000 [2]浙江大学动物科学学院,浙江杭州310058

出　　处：《中国畜牧杂志》2014年第7期31-35,共5页Chinese Journal of Animal Science

基　　金：国家自然科学基金项目(31101713);浙江省科技厅项目(2012C22067);教育部高等学校博士学科点专项科研基金(2010010112 0085);中央高校基本科研业务费专项资金资助(2012XZZX003-1)

摘　　要：为了研究绿茶多酚(GTPs)对奶牛卵母细胞体外受精和植前胚胎早期发育的影响,分别在卵母细胞体外受精(IVF)和IVF胚胎培养期间向培养液中加入不同浓度的(0、10、15、20、25μmol/L)GTPs。结果表明:在IVF培养液中添加不同浓度的GTPs均不能提高卵裂率及囊胚率;与对照组相比,添加15μmol/L GTPs到胚胎培养液中显著提高了囊胚率(33.6%vs 24.8%),但添加更高浓度的GTPs(20μmol/L或25μmol/L)并没有进一步增加囊胚率;与对照组相比,添加15μmol/LGTPs到胚胎培养液中显著提高了第7天囊胚内的GSH含量(8.2pmol/枚vs5.7pmol/枚)和GR基因的表达水平(0.39 vs 0.12),但添加更高浓度(25μmol/L)GTPs反而降低了囊胚内GSH含量和GR基因的表达。结果提示,在胚胎体外培养液中添加适量浓度的GTPs可缓解氧化应激对体外胚胎的损伤,显著提高胚胎的早期发育能力。The objective of this study was to examine the effects of green tea polyphenols （GTPs） on in vitro fertilization （IVF） and early embryo development in cow. The different concentrations of GTPs （0, 10, 15, 20 and 25 μmol/L） were added respectively into media during IVF and in vitro culture （IVC） of embryos. The results show that the cleavage rate and blastocyst rate were not significant difference when GTPs were added in IVF media. Addition of 15 μmol/L GTPs to IVC media significantly increased the blastocyst rate （33.6% vs 24.8%）, but no further increase in the blastocyst rate when higher concentrations GTPs （20 or 25 μmol/L） were added. Compared with the control group, addition of 15 μ mol/L GTPs to IVC media significantly increased the GSH content （8.2 pmol/embryo vs 5.7 pmol/embryo）and GR gene expression levels （0.39 vs 0.12）within 7 d blastocyst, but supplementation of a higher concentration （25μmol/L） GTPs reduced the GSH content and GR gene expression. These results suggested that addition of appropriate concentration of GTPs can improve the ability of early embryonic development by alleviating oxidative stress during IVC period.

关 键 词：GTPs 卵母细胞 胚胎 囊胚发育率 奶牛 

分 类 号：S823.3[农业科学—畜牧学]