七氟醚对发育中大鼠海马神经元凋亡的影响及其与细胞内钙离子相关性的研究  

The effect of sevoflurane on developing brain and its relationship with cytosolic calcium concentration

作　　者：镇路明[1] 熊虹飞[1] 薛荣亮[1] 

机构地区：[1]西安交通大学第二附属医院麻醉科,710004

出　　处：《国际麻醉学与复苏杂志》2014年第4期289-293,共5页International Journal of Anesthesiology and Resuscitation

基　　金：国家自然科学基金（81071070）

摘　　要：【摘要】目的研究七氟醚对发育中大鼠海马神经元凋亡的影响以及凋亡与细胞内钙离子的关系。方法48只1月龄SD大鼠采用随机数字表法随机分为3组（每组16只）：空气对照组（Sa组），载气对照组（Sc组）和3％七氟醚吸入4h组（s4组）。建立七氟醚吸入模型，吸入结束后12h断头取海马组织，苏木精-伊红（hematoxylin-eosin，HE）染色观察海马神经元形态学改变，用流式细胞仪检测凋亡，激光共聚焦显微镜测神经元细胞内游离钙离子浓度。结果七氟醚吸入组海马凋亡神经元数目（84．8±7．5）明显高于对照组[sa组：（1．8±1．0）；Sc组：（2．2±1．0）]，神经元内游离钙离子浓度和钙离子荧光像素值[（297±21），（373±70）]均高于对照组[Sa组：（83±13），（113±57）；Sc组：（88±18），（120±50）]，差异有统计学意义（P〈0．05）。结论七氟醚可诱导幼龄大鼠海马神经元凋亡，可能是通过升高细胞内钙离子浓度而触发凋亡。Objective To explore if sevoflurane could induce neuronal apoptosis and its relationship with intracellular calcium. Methods In this study, 48 fasted male Sprague-Dawley rats were randomly assigned into three groups. Group Sa, rats were administered with air. Group Sc, rats were administered with 100% oxygen. Group $4, rats were administered with 3% sevoflurane for 4,12 h after administration with air, oxygen or sevoflurane, all rats were killed and the hippocampus were collected for study. Cell morphology of hippocampal neuron was observed with hematoxylin-eosin （HE） staining. The percentage of apoptotic cells was measured by flow cytometry and intracellular calcium concentration was measured with laser scanning confocal microscope. Results The percentage of apoptotic neuron in hippocampus in group S4 （84.8±7.5） was higher than that in group Sa （1.8±1.0） and group Sc （2.2±1.0）（P〈O.05）. Intracellular free calcium concentration and fluorescence intensity was higher in group S4 [ （297±21）, （373±70）] than in group Sa[（83±13）,（113±57）] and group Sc[（88±18）,（120±50）] （P〈O.05）. Conclusions Sevoflurane could induce hippocampal neuron apoptosis with a potential mechanism of increasing intracellular calcium.

关 键 词：七氟醚 发育中大脑 流式细胞仪 细胞内钙离子 激光扫描共聚焦显微镜 

分 类 号：R614[医药卫生—麻醉学]