红芪水提物对急性肺损伤模型大鼠的保护作用  

Protective Effect of Water Extract of Hedysaryum polybotrys on the Acute Lung Injury Model Rats

作　　者：向红[1] 王雅莉[1] 邱桐[1] 金尧[2] 杨雪芳[1] 

机构地区：[1]甘肃中医学院病理学教研室/甘肃省中药药理与毒理学重点实验室,兰州730000 [2]甘肃中医学院中药资源教研室,兰州730000

出　　处：《中国药房》2014年第15期1352-1354,共3页China Pharmacy

基　　金：甘肃省教育厅科研项目(No.1106B-04);甘肃中医学院中青年科研基金项目(No.ZQ2011-6)

摘　　要：目的:研究红芪水提物对急性肺损伤模型大鼠的保护作用。方法:腹腔注射内毒素(LPS)5 mg/kg以复制大鼠急性肺损伤模型。60只Wistar大鼠随机分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、地塞米松(10 mg/kg)组与红芪水提物高、中、低剂量(5.2、2.6、1.3 g/kg)组,复制模型4 h后灌胃给药,每天1次,连续3 d。光学显微镜观察大鼠肺组织病理形态学变化,计算肺系数(LI)、左肺湿/干质量比(W/D)与肺含水率,免疫组化法检测肿瘤坏死因子(TNF)-α和白细胞介素(IL)-10蛋白的表达。结果:与正常对照组比较,模型组大鼠LI、W/D及肺含水率升高,IL-10蛋白表达减弱,TNF-α蛋白表达增强,差异有统计学意义(P<0.01),且肺组织病理损伤加重;与模型组比较,红芪水提物高、中、低剂量组大鼠LI、W/D及肺含水率降低,IL-10蛋白表达增强,差异有统计学意义(P<0.01或P<0.05);红芪高、中剂量组大鼠TNF-α蛋白表达减弱,差异有统计学意义(P<0.01),且大鼠肺组织病理损伤指标改善。结论:红芪水提物能明显减轻LPS所致大鼠急性肺损伤的炎症反应,其作用机制与下调TNF-α表达、促进IL-10表达有关。OBJECTIVE： To the protective effect of water extract of Hedysaryum polybotrys on the acute lung injury model rats. METHODS： ALI model was induced by intraperitoneal injection of endotoxin （LPS） 5 mg/kg. 60 Wistar rats were randomly divided into normal control group （constant volume of normal saline）, model group （constant volume of normal saline）, dexameth- asone group （10 mg/kg）, H. polybotrys high-dose, medium-dose and low-dose groups （5.2,2.6, 1.3 g/kg）. They were given relevant medicines intragastrically after modeling, once a day, for consecutive 3 days. Light microscope was used to observe histologi- cal changes, lung index （LI）, wet/dry weight ratio （W/D） and moisture content of lung were all calculated. The expressions of TNF-ct and IL-10 protein were determined using immunohistochemical method and image analysis system. RESULTS： Compared with normal control group, LI, W/D, moisture content of lung and the expression of TNF-α protein in model group were increased, while the IL-10 expression of protein was decreased; there was statistical significance（P〈0.01）; pathological changes of ALI were aggravate. Compared with model group, LI, W/D, moisture content of lung in H. polybotrys high-dose, medium-dose and low-dose groups were dereased significantly, while the IL-10 expression of protein was decreased; the expression of TNF-a in H. polybotrys high-dose and medium-dose groups was decreased significantly, there was statistical significance （P〈0.01, P〈0.05） ; pathological changes of ALI were relieved. CONCLUSIONS： Water extract of H. polybotrys could alleviate endotoxin-induced ALI rats, and its pharmacological mechanism may be related to down-regulation of TNF-α protein expression and up-regulation of IL-10 protein expression.

关 键 词：红芪水提物 急性肺损伤 肿瘤坏死因子① 白细胞介素10 

分 类 号：R285[医药卫生—中药学]