ANXA2对肝癌细胞F-actin表达及细胞微结构的调节  

Effects of ANXA2 on F-actin expression and cyto-structure of hepatocellular carcinoma cell

作　　者：武浩杰[1] 高宁[1] 何慧敏[1] 王绮轩[1] 胥谨慧[1] 王嫣然[1] 贾良杰 胡妍妍[1] 王晓静[1] 刘雨恩[1] 宋喜贵[1] 侯颖春[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2014年第2期73-76,共4页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：陕西省自然科学基金资助项目(SF2009178)

摘　　要：为了研究ANXA2对人肝癌细胞F-actin表达的调节,通过siRNA技术抑制肝癌细胞SMMC-7721的ANXA2表达,以免疫荧光法观察了F-actin表达及细胞微结构的变化.结果显示:抑制ANXA2基因表达后,细胞聚合成束的F-actin明显减弱,并由聚集分布变成弥散分布,且细胞的伪足、微绒毛等微结构生长受到抑制.结果提示,ANXA2表达水平的变化可能改变了SMMC-7721细胞F-actin的表达、聚合、重排与分布,从而导致细胞的细胞骨架重建,进而引起细胞的运动力与形态发生一系列变化.上述结果对进一步阐明ANXA2如何影响F-actin的动态组装以及ANXA2在细胞骨架系统重塑过程中的作用机制等提供了证据.Annexin A2 （ANXA2） is excessively expressed in hepatocellular carcinoma cells.To study the effects of the ANXA2 expression on the distribution of F-actin and the remodeling microstructure in human hepatocellular carcinoma cells,ANXA2 expression in SMMC-7721 cells was inhibited using RNAi method,then the expression,polymerization,distribution of F-actin were detected,observed using immunofluorescence assay.The results showed that the inhibition of ANXA2 expression weakens the expression and distribution of F-actin significantly.Additionally,the microstructures,such as pseudopodia and filopodia,associated with cell motility can be significantly remodeled and reduced.The results offer the mechanism that the ANXA2 affects F-actin expression and rearrangement,and remodels the microstructures in SMMC-7721 cells.

关 键 词：膜联蛋白A2 基因功能 肝癌细胞 细胞结构 肌动蛋白 细胞骨架 

分 类 号：Q786[生物学—分子生物学]