佩连麻黄方对单纯性肥胖大鼠减肥作用的实验研究  

Losing Weight Effect of Peilian Mahuang Prescription on Obesity Rats

作　　者：马建 孙丹[2] 赵娜 

机构地区：[1]黑龙江中医大学附属第一医院,哈尔滨150040 [2]黑龙江中医药大学,哈尔滨150040

出　　处：《中国中医药科技》2014年第2期132-134,共3页Chinese Journal of Traditional Medical Science and Technology

摘　　要：目的:观察中药佩连麻黄方对单纯性肥胖大鼠的减肥作用,探讨其作用机制。方法:将60只雄性SD大鼠随机分为5组,正常对照组,模型对照组,高、中、低剂量佩连麻黄方组,采用喂饲高脂饲料复制单纯性肥胖模型,分组给药8周。分别测定大鼠体重、体长,计算Lee’s值,采用酶法测定血清总胆固醇(TC)、甘油三酯(TG),采用双抗体夹心酶联免疫法(ELISA)测定大鼠炎性细胞因子(TNF-α、IL-6、CRP)、脂多糖(LPS)水平,采用细菌培养法测定大鼠优势肠道菌群水平。结果:与模型对照组比较,中药高、中剂量组大鼠TG、TC、Lee’s指数明显降低(P<0.01);LPS、TNF-α、IL-6、CRP明显降低(P<0.01);乳酸杆菌属、拟杆菌属、双歧杆菌属、肠球菌属均显著增加,肠杆菌属显著减少(P<0.01)。结论:佩连麻黄方可能是通过调节大鼠肠道菌群分布,减少机体慢性炎症的发生,改善机体代谢紊乱而对单纯性肥胖大鼠起到有效的减肥作用。Objective：To observe the effect of Peilian mahuang prescription on obesity rats,and explore the possible mechanism of action.Methods：60 male SD rats,divided into 5 groups randomly,the normal control group,model control group,high,medium and low concentration Peilian mahuang prescription groups,to build the model of obesity with high fat forage feeding,each group treats for 8 weeks.Weight,body length and Lee＇s were calculated,TNF-α、IL-6、GRP and LPS levels were determined by ELISA,TG and TC were examined and intestinal microflora was observed.Results：To compared with the model control group,TG,TC and Lee＇s levels of medium and high concentration groups were significantly decreased（P ＜0.01） ; LPS,IL-6,CRP levels were significantly decreased（P ＜ 0.01）; lactobacillus,bacteroides,bifidobacteria and enterococus levels were significantly increased,and enterobacter was significantly decreased（P ＜ 0.01）.Conclusions：Peilian mahuang prescription can lose weight effectively,which maybe related to adjust the distribution of intestinal microflora,and reduce chronic inflammation,and improving metabolic disturbance of body.

关 键 词：佩连麻黄方 单纯性肥胖 肠道菌群 炎性因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]