大鼠睾丸扭转/复位引发CD4＋T细胞亚群失衡对生殖细胞凋亡的影响  

Effects of unbalance CD4＋T cell subgroups on germ cell apoptosis during rat testicular torsion

作　　者：杨奇盛[1] 赵剡[1] 周贤龙[1] 倪绍洲[1] 沈俊[1] 王翔[1] 潘正启[1] 宋小兵[1] 

机构地区：[1]武汉大学中南医院急救中心,武汉430071

出　　处：《中国男科学杂志》2014年第2期11-13,共3页Chinese Journal of Andrology

基　　金：湖北省自然科学基金（项目号2011CHB026）

摘　　要：目的：探讨在大鼠睾丸扭转/复位过程中CD4＋T细胞亚群Th1/Th2失衡对生殖细胞凋亡的影响。方法 SD雄性成年大鼠30只，随机分成假手术组、睾丸扭转组和复位组，每组10只。通过建立左侧睾丸扭转和扭转复位动物模型，在手术后24h分别取材，血清酶联免疫法（ELISA）检测血清干扰素γ（IFN-γ）、白细胞介素12（IL-12）、白细胞介素4（IL-4）和白细胞介素10（IL-10）水平；原位缺口末端标记法（TUNEL法）检测各组左侧睾丸生殖细胞凋亡指数。结果与假手术组相比，扭转组和扭转复位组与Th1细胞相关的细胞因子IFN-γ、IL-12水平升高明显（P＜0.05），Th2细胞相关的细胞因子IL-4和IL-10水平下降（P＜0.05）；生殖细胞凋亡指数增加。结论在大鼠睾丸扭转和复位过程中CD4＋T细胞亚群偏移与生殖细胞凋亡存在相关性。Objective To investigate the effects of the unbalance of CD4＋T cell subgroups on germ cell apoptosis during rat testicular torsion. Methods Male SD rats were randomly divided into three groups and there were 10 rats in each group. For rats in sham operation group, they served as the control;For rats in left unilateral testicular torsion group, their left testes were rotated 720 degree for 2 hours;For rats in left testicular torsion reset group, their testes were reseted after 2 hours testicular torsion. At 24 hours after operation, levels of serum cytokines IFN-γ, IL-12, IL-4 and IL-10 were measured by ELISA. Germ cell apoptosis index in left testes of the rats was determined by TUNEL. Results Compared with that of the sham operation group, the levels of Th1 type cytokines were significantly increased in torsion and detorsion groups（P〈0.05）. The levels of Th2 type cytokines such as IL-4, IL-10 were obviously lower in torsion and detorsion groups than that in sham operation group （P〈0.05）. CD4＋T cell subgroups was unbalance during testicular torsion in rats. The apoptosis index of germ cells was significantly higher in torsion and detorsion groups than that of sham operation group. Conclusion CD4＋T cell subgroups is unbalance in ischemia reperfusion injury in rat testis, which may be associated with germ cell apoptosis.

关 键 词：T淋巴细胞亚群 精索扭转 再灌注损伤 细胞凋亡 

分 类 号：R697.2[医药卫生—泌尿科学]