曲美他嗪对大鼠放射性心肌损伤后TNF-α表达的影响  

Effects of Trimetazidine on the Expression of TNF-α in Ratsduringradiogenic Myocardial Damage

作　　者：周游[1] 陈绘颖 王江友[1] 刘涛[1] 苏强[1] 刘阳春[1] 李浪[1] 

机构地区：[1]广西医科大学第一附属医院心内科广西心血管病研究所,广西南宁530021

出　　处：《现代生物医学进展》2014年第10期1821-1824,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81160046)

摘　　要：目的:放射性心肌损伤是纵膈及胸腔等部位恶性肿瘤放射治疗后发生的严重并发症,目前尚无有效防治方法,而本实验拟探索曲美他嗪对放射性心肌损伤的保护作用。方法:将45只SD大鼠随机分为正常对照组(C组)、单纯照射组(X组)、曲美他嗪治疗组(T组),每组15只,其中X组、T组予以10 Gy/d,共3天。大鼠照射完毕处死后测定血清cTnI浓度,心肌组织TNF-αmRNA及蛋白的表达。结果:1.X组与C组相比,血清cTnI浓度,心肌组织TNF-αmRNA及蛋白的表达均明显升高(P<0.05);2.T组与C组相比,血清cTnI浓度,心肌组织TNF-αmRNA及蛋白的表达均明显升高(P<0.05);3.T组与X组相比,血清cTnI浓度,心肌组织TNF-αmRNA及蛋白的表达均明显降低(P<0.05)。结论:曲美他嗪能够通过减少心肌组织TNF-αmRNA及蛋白的表达,从而有效减轻大鼠放射后心脏炎症反应,以减轻心肌损伤。Objective： Radiogenic myocardial damage is a serious complication of mediastinal and pleural and other parts of malignant tumor after radiotherapy, there is no effective control methods, and in this study intends to explore the protective effect of- trimetazidine on radiogenic myocardial damage. Methods： 45 SD rats were randomly divided into normal control group （group C）, irradiation alone group（group X）, trimetazidine treatment group （T group）, 15 rats in each group, X group. T group were given 10 Gy/d, a total of 3 days, a total dose of 30 Gy X-ray irradiation. Irradiated rats were completed by measuring the concentration of serum cTnI, the expression of TNF- α protein and mRNA in myocardial tissue. Results： 1.Compared with X group and C group, the concentration of serum cTnI, the expression ofTNF- α protein and mRNA in myocardial tissue were significantly increased （P〈0.05）; 2. Compared with T group and C group the concentration of serum cTnI, the expression of TNF-α protein and mRNA in myocardial tissue were significantly increased （P〈0.05）; 3.compared with T group and X group, the concentration of serum cTnI, the expression of TNF-α protein and mRNA in myocardial tissue were significantly decreased （P〈0.05）. Conclusions： Trimetazidine can reduce the expression of TNF- mRNA and proteinin cardiac tissue, so as to effectively reduce the inflammatory response in heart tissue in rats after radiation, to reduce myocardial injury.

关 键 词：放射性心脏损伤 曲美他嗪 TNF-Α 大鼠 TNF-Α 

分 类 号：Q255[生物学—细胞生物学] R541.9[医药卫生—心血管疾病]