有氧运动对非酒精性脂肪肝大鼠肝细胞PPARα mRNA表达的影响  

Influence of Aerobic Exercise on Expression of PPARα mRNA in Rats with Nonalcoholic Fatty Liver Disease

作　　者：杨文吉[1] 崔旭妍[2] 郑晟琳 饶志坚[1] 袁海平[1] 

机构地区：[1]上海体育学院运动科学学院,上海200438 [2]天津医学高等专科学校医疗技术系,天津300000

出　　处：《现代生物医学进展》2014年第10期1855-1860,共6页Progress in Modern Biomedicine

基　　金：上海体育学院研究生创新基金项目(S1101340);上海体育学院省部级重点实验室开放基金项目(S6401209);上海市人类运动能力开发与保障重点实验室(上海体育学院)(NO.11DZ2261100)

摘　　要：目的:研究有氧运动对非酒精性脂肪肝大鼠肝细胞PPARα表达的影响,探讨运动对非酒精性脂肪肝的保护防治作用。方法:36只雄性SD大鼠随机分为对照组,高脂组和高脂运动组。后两组喂食普通饲料的同时在实验前三周给予脂肪乳剂灌胃,以诱发脂肪肝。七周有氧运动后测大鼠体重、肝湿重、测血浆TC、TG、ALT、SOD及MDA水平;采用荧光定量PCR法测肝细胞PPARαmRNA的表达水平。结果:1)与对照组相比,高脂组TC、TG显著升高(P<0.05,P<0.01),高脂运动组均无显著性差异。高脂组TC、TG显著高于高脂运动组(P<0.01,P<0.01)。2)高脂组PPARαmRNA表达显著低于对照组(P<0.05)和高脂运动组(P<0.01);高脂运动组显著高于对照组。3)高脂组肝小叶结构模糊,肝窦界限不清,汇管区结构模糊,肝细胞肿胀,有空泡样改变,肝脂肪变性。高脂运动组肝脏仍有脂肪积聚,但肝细胞空泡消失,呈好转趋势。4)高脂运动组MDA显著低于高脂组(P<0.01);高脂运动组SOD显著高于高脂组(P<0.01)。结论:运动可以通过上调PPARαmRNA来促进脂肪酸氧化、降低血脂,从而有效预防肝细胞脂肪变性。Objective： To investigate the influence of aerobic exercise on the expression of PPARotin rats with nonalcoholic fatty liver disease. Methods： Male SD rats weighting 160-200g were randomly divided into three groups： control group （N）： the rats in N group were fed with standard diet; high fat group （H）： the rats in H group were fed with a standard diet plus fat emulsion during the first 3 weeks for inducing fatty liver and then with standard diet; and exercise with high fat group （HE）： the rats in HE group were fed with same diet regimen as that for rats in H group, but they were given a daily load of 90 min swimming. After 7 week experiment, the livers were procured and weighted for calculating liver weight index, and the expression level of PPARa in the livers was measured by fluorescence quantitative PCR. The serum was separated for detecting TC, TG, MDA, SOD and ALT. Results： 1） The expression ofPPARa mRNA in rats at H group was significantly lower than that in rats at N group （P〈0.05） and HE group （P〈0.01）. 2） The concentrations of TC and TG in the rats at H group were significantly higher than that in the rats at N group and HE group （P〈0.01）, while they were not significantly different between HE group and N group. 3）In H group, hepatic lobule structure fuzzy, hepatic sinusoid boundary unclear, hepatic portal structure fuzzy, hepatocyte swelling and become lipid vacuoles, hepatic steatosis. In the group of HE, the lipid still accumulate in liver, but lipid vacuoles disappeared and showed signs of improvement. 4） The serum content of MDA in rats at HE and N groups were significantly higher than that in rats at H group （P〈0.01）, while the serum content of SOD in rats at HE and N groups were significantly lower than that in rats at H group （P〈0.01）. Conclusions： Up regulation of expression of PPARct gene by aerobic exercise can attenuate fatty degeneration in hepatic cells.

关 键 词：非酒精性脂肪肝 PPARΑ 有氧运动 

分 类 号：S852.4[农业科学—基础兽医学] R575.5[农业科学—兽医学]