HPLC法测定异黄酮类化合物BI的含量及有关物质  

Determination of the Content and the Related Substances of Isoflavone Compounds,BI

作　　者：江玉莲[1,2] 杨建云[2] 肖炳坤[2] 黄荣清[2] 

机构地区：[1]江西中医药大学,南昌330004 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《现代科学仪器》2014年第1期101-104,共4页Modern Scientific Instruments

基　　金：基金项目：国家自然科学基金（NO.81072613）;十二五国家重大专项（NO.2012ZX09301003-001-010）国家“重大新药创制”科技重大专项（NO.2013ZX09102-016）

摘　　要：目的：建立测定异黄酮类化合物BI的含量及有关物质的高效液相色谱方法。方法：采用AgilentZorbaxescilpseXDBC18（250mm×4．6mm，5．0μm）色谱柱；以0．15％醋酸-甲醇（45：55）为流动相；流速：1．OmL／min；检测波长为260nm；柱温40℃；进样体积：10μL。结果：在上述色谱条件下，各杂质及各降解产物均可与BI主峰良好分离，HPLC测定的线性范围为0．08μg-0．80μg（r=0．9999），方法的仪器精密度、日内精密度、日间精密度及重复性的RSD均小于2％。结论：采用HPLC测定中药BI的含量及有关物质，方法简便，结果准确可靠。Objective：To establish an RP-HPLC method for determination of the content and the related substances of Isoflavone compounds,BI.Methods：The separation was performed on an Agilent Zorbax escilpse XDB C18 column （250mm ×4.6mm,5.0μm）;the mobile phase consisted of methanol-0.15%acetic acid （55：45） at the flow rate of 1.0 mL/ min;the detective wavelength was set at 260nm;the column temperature was 40 ℃;and the injection volume was set at 10μL.Results：An excellent separation was achieved for BI and its related substances.The assay was validated in the concentration range of 0.08μg-0.80μg （r=0.9999）.All of the instrument precision,precision in a day, precision during the days and the repeatability were less than 2%.Conclusion：The developed method can give more accurate and sensitive results for the determination of the content and the related substances of BI.

关 键 词：BI 含量测定 有关物质 高效液相色谱法 

分 类 号：TH833[机械工程—仪器科学与技术]