microRNA-34c在不同发育时期雄性小鼠脑组织中的表达分析  

Expression of microRNA-34c in male mice brain tissues at different developmental stages

作　　者：刘笙腩[1] 于萌[1] 颜润川 张伟[1] 段明慧[1] 胡新德[1] 华进联[1] 陈树林[1] 赵善廷[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2014年第3期39-44,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：西北农林科技大学人才引进专项基金项目(Z111021101);国家"863"高技术研究发展计划项目(2011AA10A208)

摘　　要：【目的】研究microRNA-34c(简称miR-34c)在出生后不同发育时期小鼠嗅球、大脑皮质、小脑皮质及海马中的表达情况,并分析其与小鼠行为活动之间的相关性。【方法】以出生后0,3,7,14,21,28,60和90d的雄性昆明小白鼠为试验动物,以5SrRNA为内参基因,运用荧光实时定量PCR技术,检测miR-34c在小鼠出生后8个不同发育时期嗅球、大脑皮质、小脑皮质和海马中的相对表达量以及在成年鼠脑组织中4个不同部位的相对表达量。【结果】随着小鼠的发育,miR-34c在嗅球、大脑皮质、小脑皮质及海马中的相对表达量均呈现不同程度的递增趋势。在成年鼠中,miR-34c在嗅球、大脑皮质、小脑皮质、海马中的相对表达量差异显著,其中海马中最高,小脑皮质次之,嗅球最低。【结论】miR-34c在出生后不同发育时期小鼠嗅球、大脑皮质、小脑皮质及海马中的表达趋势,与小鼠各种行为活动的出现具有一定的相关性。[Objective] This study aimed to investigate the expression of microRNA-34c （miR-34c） in olfactory bulb,neocortex, cerebellar cortex and hippocampus of male mice at different developmental stages after birth,and explore its relationship with mice behaviors. [Method] Using Kunming male mice at differ- ent ages （including 0,3,7,14,21,28,60 and 90 d） as experimental animals, and 5S rRNA as the internal gene,florescent real-time quantitative RT-PCR was used to detect relative expression levels of miR-34c in olfactory bulb,neocortex, cerebellar cortex and hippocampus at eight different developmental stages after birth and in four different tissues of adult mice. [Result] With the development of mice,expression levels of miR-34c in olfactory bulb, neocortex, cerebellar cortex and hippocampus all increased. Expression levels of miR-34c in the four tissues of adult mice were significant different. The level in hippocampus was the highest followed by cerebellar cortex,while the expression in olfactory bulb was the lowest. [Conclusion] The different expression levels of miR-34c in olfactory bulb, neocortex, cerebellar cortex and hippocampusat different developing stages after birth were related to the behaviors of mice.

关 键 词：miR-34c 小鼠 脑组织 实时定量PCR 

分 类 号：S865.131[农业科学—野生动物驯养] Q786[农业科学—畜牧兽医]