五紫三黄方对激素依赖性哮喘小鼠模型嗜酸性粒细胞趋化活化因子和嗜酸性粒细胞阳离子蛋白的影响研究  

Effect of Wuzi Sanhuang Recipe on Eotaxin and Eosinophil Cationic Protein( ECP) in Hormone Dependent Asthma Mice

作　　者：苏奎国[1] 姜良铎[2] 郭勇英[3] 

机构地区：[1]河北省中医院呼吸内科,河北石家庄050011 [2]北京中医药大学东直门医院,北京100700 [3]河北医科大学

出　　处：《四川中医》2014年第3期65-67,共3页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:观察五紫三黄方对激素依赖性哮喘小鼠模型嗜酸性粒细胞趋化活化因子和嗜酸性粒细胞阳离子蛋白的影响。方法:小鼠雌雄各半120只,随机分为空白组、模型组、泼尼松对照组及五紫三黄方低、中、高剂量组,每组20只。利用卵蛋白(OVA)复制小鼠哮喘模型,并进行激素干预治疗来制作激素依赖性哮喘小鼠模型,在撤减激素过程中,以五紫三黄方干预治疗,观察其对哮喘小鼠模型嗜酸性粒细胞趋化活化因子和嗜酸性粒细胞阳离子蛋白的影响。结果:五紫三黄方各组与醋酸泼尼松均能减少肺组织内ECP和eotaxin的含量(P<0.01),五紫三黄方减少肺组织内ECP和eotaxin的含量较醋酸泼尼松显著(P<0.01)。结论:五紫三黄方具有很好的减轻气道炎症的作用。Objective： To observe the effect of eotaxin and eosinophil cationic protein （ECP） on hormone dependent asth- ma mice with Wuzi Sanhuang Recipe. Methods： 120 rats （half male and female） were randomized divided into six groups （blank group, model group, prednisone group, Wuzi Sanhuang Recipe low dose group, middle dose group and high dose group, 20 mice in each group）. Duplicating the asthma model in mice with egg protein （OVA） and make the hormone depend- ent asthma model with hormone intervention treatment. In the process of subtract hormone, do the intervention treatment with Wuzi Sanhuang Recipe. And observing the effect of eotaxin and ECP. Results： Groups dealt with different doses of Wuzi San- huang Recipe and prednisone could reduce the content of ECP and eotaxin in lung tissue （P 〈0. O1 ） and effects of the former was superior to that of prednisone （P 〈0. 01 ）. Conclusion： Wuzi Sanhuang Recipe has good effect of alleviating the airway in- flammation.

关 键 词：五紫三黄方 哮喘小鼠 趋化活化因子和阳离子蛋白 实验研究 

分 类 号：R285.5[医药卫生—中药学]