黑荆树皮单宁不同级分对水解酶的抑制作用  

INHIBITORY EFFECTS OF DIFFERENT FRACTIONS OF BLACK WATTLE BARK TANNINS ON HYDROLASES

作　　者：姚开[1] 吕远平[1] 石碧[1] 何强[1] 

机构地区：[1]四川大学轻工与食品工程学院,四川成都610065

出　　处：《林产化学与工业》2000年第4期5-10,共6页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金"具有生物活性的单宁化合物及其磷酸酯衍生物的合成! ( 2 9872 0 2 5)"资助项目

摘　　要：通过对黑荆树皮单宁进行醇沉纯化和溶剂萃取分级 ,可得到分子量分布范围不同的单宁级分。各级分对 5种水解酶均表现出不同程度的抑制作用 ,具有一定的选择性。其抑制能力与抑制剂常数Ki 值有关 ,分子量大的单宁级分 ,Ki 值小 ,对酶活性的抑制作用大 ,同时 。Tannin fractions with different molecular weight distributions were obtained by purification of alcohol preci pitation and fractionation of solvent extractions of black wattle bark tannins. The data observed in the experiments indicated that tannin fractions possess varying inhibitory effects on activity of hydrolases, showing selective inhibition. Based on comprehensive investigations of cellulase, it was displayed that the inhibitions are related to the inhibitor constant K i . When the molecular weight of the fractions is higher, K i value is lower and the inhibitory effect is stronger. Meanwhile, the inhibition of the fractions on the cellulase was proved to be a noncompetitive one.

关 键 词：植物单宁 黑荆树皮单宁 分级 水解酶 抑制作用 天然鞣料 

分 类 号：TQ943.2[化学工程] Q556[生物学—生物化学]