兔死后角膜内皮细胞活性率变化的时间规律性研究  

The changes of living rate of cornea endothelial cells 72h after the death of rabbits

作　　者：李晓娜[1] 郑吉龙[2] 单迪[2] 徐恩宇[1] 

机构地区：[1]中国医科大学基础医学院化学教研室,辽宁沈阳110001 [2]中国刑事警察学院法医系法医病理教研室,辽宁沈阳110035

出　　处：《解剖科学进展》2014年第2期115-118,共4页Progress of Anatomical Sciences

基　　金：沈阳市科技计划项目(F13-182-9-00)

摘　　要：目的研究兔死后72h内角膜细胞形态学变化规律与死亡时间的关系,从细胞水平和分子水平两个角度探讨个体死亡后角膜细胞的一般变化规律,为早期死亡时间的推断提供新的方法。方法用空气栓塞的方法建立兔死亡模型。在兔死后0-72h,每隔6h进行角膜取材,采用茜素红-曲利苯兰联合染色法对角膜内皮细胞进行染色,显微镜下摄像,计算角膜内皮细胞活性率(Endothelial cell living rate,ECLR),并作统计学分析。结果茜素红-曲利苯兰联合染色结果显示:个体死后6h,角膜内皮失活细胞数目开始增加,死后72h,失活细胞数目达到高峰。角膜内皮细胞活性率随着死后间隔时间的延长而逐渐下降,其与死亡时间(Postmortem Interval,PMI)存在着较强的负相关。结论个体死后72h内,采用细胞形态学方法结合Motic Images Plus 2.0图像分析软件检测角膜内皮细胞活性率变化规律,可用于早期PMI的推断。Objective To study the relationship between the morphology changes of corneal endothelial cells with the postmortem interval（PMI） in rabbits to find a new method for estimating PMI by detecting the changes of endothelial cell living rate（ECLR）. Methods A rabbit death model was established by air embolism. The corneal endothelial cells were taken out at every 6-hour-interval from 0 hours to 72h respectively after the rabbit death. The sections were stained by alizarin red-benzene Trypan blue, and ECLR was calculated under the optical microscope and analyzed statistically. Results The alizarin red-benzene Trypan blue staining showed that the number of the dead corneal endothelial cells began to increase at 6h after death and reached the peak at 72h after death. The endothelial cell living rate declined as the increase of PMI, and correlated negatively with PMI. Conclusion The combination of cell morphology by detecting the changes of ECLR with Motic Images Plus 2.0 image analysis software might be useful for the estimation of early postmortem interval within 72 hours after dead.

关 键 词：法医病理学 死后间隔时间 角膜内皮细胞 形态学 免 

分 类 号：D919.1[医药卫生—法医学]