勿动蛋白受体对糖尿病大鼠视网膜神经节细胞的影响  

Effect of NgR on the retinal ganglion cells in diabetic rats

作　　者：单伟[1] 蒋丽华 王岩 李德华[1] 刘学[1] 崔楠 左中夫[1] 

机构地区：[1]辽宁医学院解剖学教研室 [2]辽河油田第二职工医院内科 [3]辽河油田总医院放射线科 [4]95905部队场站卫生队

出　　处：《解剖科学进展》2014年第2期131-133,共3页Progress of Anatomical Sciences

基　　金：国家自然科学基金(No.81300931);辽宁省自然科学基金(No.2013022055);辽宁省教育厅课题(No.L2013332)

摘　　要：目的探讨勿动蛋白受体(nogo receptor,NgR)在糖尿病(diabetes mellitus,DM)SD大鼠视网膜神经节细胞凋亡中的作用及可能机制。方法链脲佐菌素诱导SD大鼠DM模型,实验分4组:对照组,DM组,siNgR组(玻璃体内给予NgR反义核苷酸序列),siRNA空白组(玻璃体内给予阴性核苷酸序列)。1个月后TUNEL染色检测视网膜神经节细胞(retinal ganglion cell,RGC)凋亡,试剂盒检测视网膜丙二醛(malondialdehyde,MDA)含量,Western blot检测视网膜NgR及caspase-3含量。结果对照组、DM组、siRNA空白组及siNgR组MDA含量分别为3.69±0.51、7.18±0.87、6.52±1.27及3.08±0.48 nmol/mg prot。与对照组相比,DM组及siRNA空白组视网膜RGC凋亡增加,NgR及caspase-3表达上调,MDA含量升高(p<0.05),而siNgR组视网膜RGC数量、NgR及caspase-3表达、MDA含量较对照组均无明显变化(p>0.05)。结论 NgR过表达是糖尿病视网膜RGC凋亡的原因之一,其机制可能与NgR诱导视网膜氧化应激、上调caspase-3表达相关。Objective To explore the role and underlying mechanisms of NgR on the retinal ganglion cell（RGC） in diabetic rats. Methods SD rats were divided into control group, diabetes mellitus（DM） group, siNgR group and siRNA control group, diabetic models were induced by administrating streptozotocin, siNgR rats were injected by anti-NgR nucleotide intravitreally, and siRNA control rats were injected by negative nucleotide intravitreally. One month later, TUNEL staining was conducted to detect apoptosis of RGCs, level of retinal malondialdehyde （MDA） was detected with MDA kit, and expressions of NgR and caspase-3 were observed by Western blot. Results The levels of retinal MDA in control group, DM group, siNgR group and siRNA control group were 3.69±0.51, 7.18 ±0.87, 6.52± 1.27 and 3.08±0.48 nmol/mg prot respectively. The number of TUNEL-positive RGCs, the expressions of retinal NgR and caspase-3, and the level of retinal MDA were significantly increased in DM and siRNA groups than in control and siNgR groups （P〈0.05）, but with no difference between control group and siNgR group （P 〉0.05）. Conclusion The retinal oxidative stress and upregulation of retinal caspase-3 induced by NgR indicate that NgR might play an important role in the apoptosis of diabetic RGC.

关 键 词：勿动蛋白受体 糖尿病视网膜病变 视网膜神经节细胞 SD大鼠 

分 类 号：R587.2[医药卫生—内分泌]