子午沙鼠子鼠肾中水通道蛋白1、2的表达  

Expression of AQP 1,2 Proteins in Kidney of Filial Meriones meridianus

作　　者：张春燕[1] 侯天德[2] 程昉[2] 桑秋[2] 

机构地区：[1]河西学院农业与生物技术学院,张掖734000 [2]西北师范大学生命科学学院,兰州730070

出　　处：《动物学杂志》2014年第2期162-169,共8页Chinese Journal of Zoology

基　　金：甘肃省教育厅研究生导师科研项目(No.0801-06);甘肃省科技计划项目(No.1304FKCG103)

摘　　要：应用免疫组织化学方法和体视学半定量方法检测了水通道蛋白1、2(aquaporin 1,2)在子午沙鼠(Meriones meridianus)子鼠肾中的表达,利用IPP专业图像分析软件对其表达强度进行了定量分析。结果显示,1、7、14、21、32日龄子午沙鼠仔鼠肾均有水通道蛋白1、2的阳性表达,AQP1表达部位为肾近曲小管上皮细胞的顶质膜和基底膜、髓袢细段;AQP2的表达位于集合管主细胞的游离面细胞膜;7日龄子午沙鼠仔鼠肾AQP1阳性表达的面密度增加显著(P<0.05),光密度增加极显著(P<0.01),7日龄子午沙鼠仔鼠肾AQP2阳性表达的面密度和光密度增加极显著(P<0.01),7日龄后无差异(P>0.05)。结果表明,7日龄后子午沙鼠子鼠肾AQP1、2的表达水平增强,近曲小管与髓袢细段、集合管对水的重吸收增强,以浓缩尿液,AQP1、2对子鼠尿的浓缩起重要作用,从而调节肾水的平衡。The expression of AQP1, 2 in kidney of filial Meriones meridianus was investigated by immunohistochemistry combined with stereological method, and the expression intensity of AQP1, 2 was analyzed by IPP professional image analysis software. Results showed that there was expression of AQP1 , 2 in kidney of filial M. meridianus at postnatal day 1, 7, 14, 21, and 32. AQP1 immunopositive cells mainly distributed in the epithelial cells of proximal convoluted tubule and thin segment of medullary loop in kidney at postnatal day 1, 7, 14, 21, and 32,while AQP2 expressed on the apical plasma membrane of collecting duct principal cells at postnatal day 1, 7, 14, 21, and 32. Surface area density and average optical density of AQP1 positive cells in proximal convoluted tubule and thin segment of medullary loop and AQP2 positive cells in collecting duct in kidney of filial M. meridianus increased （ P 〈 0.05 or P 〈 0. 01 ） at postnatal 7 day, but there was no significant difference from postnatal day 7 to postnatal day 32 （ P 〉 0. 05 ）. The results showed that the expression level of AQP1, 2 positive cells in kidney of filial M. meridianus increased at postnatal day 7, when the ability of water reabsorption of proximal convoluted tubule, thin segment of medullary loop and collecting duct increased. AQP1, 2 might play an important role in urine concentrating of filial M. meridianus kidney to regulate the balance of water in kidney.

关 键 词：子午沙鼠子鼠 .肾发育 水通道蛋白1 2表达 免疫组织化学 

分 类 号：Q51[生物学—生物化学]