丹参干预HHcy兔的心室重塑作用  

Effect of Salvia Miltiorrhiza on Ventricular Remodeling in Hyperhomocysteinemia Rabbits

作　　者：武跃明[1] 康爱英[1] 

机构地区：[1]河南省南阳医学高等专科学校,河南南阳473058

出　　处：《中国伤残医学》2014年第7期27-29,共3页Chinese Journal of Trauma and Disability Medicine

摘　　要：目的:研究丹参对高同型半胱氨酸血症(HHcy)兔心室重塑的影响。方法:用2%蛋氨酸饮食制备兔HHcy模型,丹参3.3 g/Kg·d灌服干预,以正常家兔为对照,8周末分别检测各组家兔血清丙二醛(MDA)、超氧化物岐化酶(SOD)、同型半胱氨酸(Hcy)、一氧化氮(NO)、内皮素(ET)和血栓素B2(TXB2)含量,处死家兔观察心脏结构病理改变。结果:实验8周时,蛋氨酸组家兔与正常组比较血清Hcy、MDA、ET、TXB2显著升高(P<0.01),NO、SOD明显降低(P<0.01);组织学及免疫组化观察心肌内血管数减少,心肌横径增大,动脉管壁显著增厚,胶原纤维及Actin-平滑肌肌动蛋白表达增强;丹参组家兔与蛋氨酸组相比血清Hcy、MDA、ET、TXB2含量显著降低,NO、SOD水平升高;心肌内血管数目明显回升,胶原纤维和肌动蛋白表达减少。结论:HHcy可诱发兔心室重塑,丹参通过抑制a-SMA的表达、逆转心肌纤维化、提高抗氧化酶的活性等机制,改善和拮抗HHcy兔的心室重塑作用。Objective： To observe the Effect of Salvia miltiorrhiza on ventrieular remodeling in hyperhomocysteinemia （HHey） rabbits. Methods： Use 2% methionine （MET） diet to establish the HHcy rabbits models, fed the experimental group with Salvia miltiorrhiza 3. 3g/kg. d for 8 weeks, Detect the content of the malonaldehyde （MDA）, the superoxide dismutase （SOD） and the nitric oxide （NO） in each group , observe the pathological changes of cardiac structure at the same time. Results： 8 weeks after the experiment, the HCY MDA and TXB2 levels in the MET group were significantly increased（ p 〈 O. 01 ）, but the level of NO and SOD were significantly de- creased （p 〈 O. 01 ） ; observe by histology and immunohistochemistry, the number of vessel in myocardium is decreased, the transverse di- ameter of myoeardium is increased, the arterial wall is significantly thickened, the express of the collagenous fiber and the smooth muscle actin were enhanced; the MDA and TXB2 levels in the salvia miltiorrhiza group were significantly decreased（ p 〈 0. O1 ）, but the level of NO and SOD were significantly increased（ p 〈 O. 01 ） ; the number of vaseellumin in myocardium is increased, the express of the collage- nous fiber and the smooth muscle actin were decreased. Conclusion ： the HHey can induce the ventrieular remodeling of rabbits, because Salvia miltiorrhiza can suppression the express of A - SMA, reverse myocardial fibrosis, increase the antioxidant enzyme activity, it can improve and rival the ventricular remodeling in HHcy rabbits.

关 键 词：丹参 干预 HHCY 心室重塑 

分 类 号：R33[医药卫生—人体生理学]