磷酸化-JNK1/2在高温处理后的鼠胚心脏的表达变化  

Expressions of p-JNK1/2 in Embryonic Heart of Hamster after Hyperthermia

作　　者：冷昭廷[1] 于丽[1] 刘文静[1] 闫雪[1] 王力[1] 付志达[1] 张帅[1] 隽兆东[1] 

机构地区：[1]潍坊医学院外科学教研室,山东潍坊261053

出　　处：《潍坊医学院学报》2014年第1期70-72,共3页Acta Academiae Medicinae Weifang

基　　金：国家自然科学基金资助课题(课题编号:30900775);山东省自然科学基金资助课题(课题编号:ZR2010CQ036)

摘　　要：目的探讨p-JNK1/2高温处理后金黄地鼠胚胎心脏之表达变化。方法将金黄地鼠孕鼠在受孕8d时置42℃水浴20min,在高温处理后12h,24h,36h,48h剖腹取胎,制备为石蜡切片。利用免疫荧光染色技术,观察心脏正常发育与异常分化过程中磷酸化JNK(p-JNK)的表达。结果 高温处理后12h,24h,36h,48h,实验组鼠胚心脏p-JNK1/2的表达较对照组均减弱,其中36h和48h两个时间点差异显著。结论 高温可致金黄地鼠胚胎心脏磷酸化JNK1/2(p-JNK1/2)表达减低,p-JNK1/2对心脏正常发育具有重要作用。Objective To explore the effects of hyperthermia on the development of hamster embryonic heart. Methods Ham- sters were ramlomly divided into 2 groups. Experimental groups were subjeeted to hyperthermia{42~C in hot water for 20rain） in day 8 post coitus. The embryos were obtained at 12,24,36,48h after treatment respectively, and serial sections of embryonic heart were prepared. The expressinns of proteins p-JNKl/2 in the embryonic heart were observed with iramunofluorescent staining technique. Results At 12,24,36 and 48 hours,expressions of p-iNK1/2 in the experimental groups were decreased, and the 36 and 48hours were decreased significantly. Con- clusion The expressions of p-JNKl/2 decrease significantly in the embryonic heart of hamster after hyperthermia,suggesting p-JNKl/2 may play an important role in the normal development of heart.

关 键 词：心脏 高温 p-JNK1 2 胚胎 

分 类 号：R446.6[医药卫生—诊断学]