舒郁散对慢性应激抑郁大鼠行为及海马神经肽表达的影响  

Effect of Shuyusan on Behavior and Neuropeptide Expression in Hippocampus with Chronic Mild Unpredictable Stressors Depression-like Rats

作　　者：陈利平[1] 王发渭[1] 孙志高[1] 许成勇[1] 

机构地区：[1]中国人民解放军总医院海南分院中医科,海南三亚572104

出　　处：《中国中医药信息杂志》2014年第4期46-48,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：军队"十二五"科研攻关项目(CWS12J129)

摘　　要：目的观察舒郁散对慢性应激抑郁大鼠的行为变化、脑组织肿瘤坏死因子(TNF-α)含量及海马神经肽表达的影响,为临床治疗提供实验依据。方法 50只大鼠随机分为正常组、模型组、盐酸氟西汀组及舒郁散小、大剂量组,各治疗组给予相应药物,正常组与模型组给予等容积蒸馏水。应激刺激造模,采用糖水消耗实验、悬尾实验观察大鼠行为改变,采用ELISA检测脑组织TNF-α含量,免疫组化法观察大鼠海马神经肽表达。结果盐酸氟西汀、舒郁散大剂量组大鼠悬尾不动时间明显减少,糖水消耗量明显增加,与模型组比较差异有统计学意义(P<0.05)。盐酸氟西汀、舒郁散大剂量组大鼠脑组织TNF-α含量显著降低,与模型组比较差异有统计学意义(P<0.05)。盐酸氟西汀组、舒郁散大剂量组海马神经肽免疫反应阳性神经元数目减少,平均光密度降低,与模型组比较差异有统计学意义(P<0.05)。结论舒郁散具有抗抑郁作用,其作用与剂量呈正相关。Objective To investigate the effect of Shuyusan on the behavior, content of tumor necrosisfactor-α（TNF-α） in cortex of cerebral and neuropeptide expression in hippocampus of rats with chronic mild unpredictable stress （CMUS） depression. Methods Fifty mice were randomly divided into 5 groups：normal group, model group, fluoxetine group, and high-, low-dose group of Shuyusan. Except normal group, the other groups were given CMUS. Tail suspend test （TST） and sucrose preference test （SPT） were used to estimate animals’ behavior. The contents of TNF-αwas determined by enzyme linked immunosorbent assay （ELISA）. The immunocytochemical technique was used to evaluate hippocampus neuropeptide immunoreactive expression. Results The fluoxetine group and high dose of Shuyusan group showed significant increase in the sucrose consumption and decrease in the immobility time in TST, there was significant difference compared with CMUS model group （P〈0.05）. The TNF-αlevel was significantly lower in fluoxetine group and high dose of Shuyusan group, there difference was significant compared with model group （P〈0.05）. The neuropeptide immunoreactive neurons in hippocampus area was decreased by a marked number in fluoxetine group and high dose of Shuyusan group, the average optical density decreased significantly, compared with the model group, there were significant differences （P〈0.05）. Conclusion Shuyusan has anti-depression likely effect, with dose-effect relationship.

关 键 词：舒郁散 抑郁症 动物行为 肿瘤坏死因子-Α 神经肽 大鼠 TNF-α 

分 类 号：R285.5[医药卫生—中药学]