川芎药材HPLC指纹图谱研究  

Study on Fingerprint of Chuanxiong Rhizoma by HPLC

作　　者：贡磊[1] 狄留庆[2] 陈凌云[1] 李杰[2] 严燕清 李全 

机构地区：[1]云南中医学院,云南昆明650500 [2]南京中医药大学药学院,江苏省中药高效给药系统工程技术研究中心,江苏南京210023 [3]雷允上(苏州)药业有限公司,江苏苏州215000

出　　处：《中国中医药信息杂志》2014年第4期86-89,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：江苏省科技成果转化资金项目(BA2012078);江苏省产学研前瞻性项目(BY2013008-01)

摘　　要：目的建立川芎药材的高效液相色谱指纹图谱。方法使用色谱柱Thermo C18(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%甲酸水溶液,梯度洗脱,柱温25℃,流速1.0 mL/min,检测波长323 nm。通过提取11个色谱峰作为指纹图谱共有峰,分别采用相似度评价,聚类分析和主成分分析等方法,对所收集的18批样品进行系统比较与归类。结果通过比较18批川芎的色谱图,确定了11个共有峰,大部分样品的相似度在0.9以上,并将不同川芎分为3大类。结论该方法重复性好,简便可靠,可以为川芎的质量控制和评价提供依据。Objective To establish analysis methods for fingerprint of Chuanxiong Rhizoma by HPLC. Methods Thermo C18 chromatographic column （4.6 mm×250 mm, 5 μm） was used with methanol-0.1% Formic acid in gradient elution. The flow rate was 1 mL/min, the detection wavelength was set at 323 nm, and the temperature was 25 ℃. The similarities of the 18 batches of samples were compared by similarity evaluation, cluster analysis and principal component analysis. Results Based on the fingerprints of 18 batches of Chuanxiong Rhizoma, 11 common peaks were identified, the similarities were almost greater than 0.9 among all batches. The samples were clustered into 3 categories. Conclusion The method is simple, steady and repeatable. It provides a basis for the quality control and evaluation of Chuanxiong Rhizoma.

关 键 词：川芎 指纹图谱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]