氮杂大环铈(III)配合物与pUC19 DNA的相互作用（英文）  

The interactions between an aza-cyclic cerium(III) complex and pUC19 DNA

作　　者：王敏[1] 俞兰[1] 李方珍 谢家庆[1] 

机构地区：[1]重庆理工大学化学化工学院,重庆400054

出　　处：《催化学报》2014年第4期524-531,共8页

基　　金：supported by the National Natural Science Foundation of China (21173274);Major Science and Technology Project of Zigong City (2013X03);the Educational Department of Sichuan Government (13ZA0126)~~

摘　　要：以一种1,4,7,10,13,16,19,22,25,28,31-十一氮杂2,5,8,11,14,17,20,23,26,29,32-十一酰基-环三十三烷的衍生物(CYC)为配体,在溶液中和Ce(Ⅲ,M)离子形成氮杂大环铈配合物(M-CYC)作为仿生催化剂,通过紫外光谱法、荧光光谱法和凝胶电泳法研究了M-CYC配合物与DNA的相互作用.结果表明,M-CYC与小牛胸腺DNA(CT DNA)以嵌插方式相互作用,在pH=8.16的缓冲体系中,其结合常数Kb=2.0×104 L/mol.凝胶电泳结果表明,当自由基捕捉剂存在时,M-CYC可将超螺旋pUC19 DNA切割为缺刻型DNA,其切割方式为水解切割.A new biomimetic catalyst （M-CYC） is formed from the combination of CYC （a derivative of 1,4,7,10,13,16,19,22,25,28,31-undecaaza-cyclotritriacontane-2,5,8,11,14,17,20,23,26,29,32-undeca acyl） and Ce（III, M） in aqueous solution. The interaction between this complex and DNA was studied using electronic absorption spectroscopy, fluorescence spectroscopy, and gel electrophoresis. The results indicated that M-CYC and calf thymus DNA （pUC19 DNA） interacted via intercalation. The apparent binding constant of the complex with DNA was determined to be 2.0x10^44 L/mol at pH 8.16. Based on gel electrophoresis analysis, M-CYC was shown to nick the circular plasmid pUC19 DNA in the presence of a free radical scavenger. The nicking of DNA serves as a model of hydrolyzed cleavage.

关 键 词：氮杂大环 铈(III) 配合物 切割 

分 类 号：O641.4[理学—物理化学]