农杆菌与质粒DNA子房注射法在棉花转基因研究中的应用  

Application of Ovary Injection with Agrobacterium tumefaciens or Plasmid DNA in Transgenic Cotton

作　　者：匡政成[1,2] 张学文[2] 肖才升[1] 陈浩东[1] 黄丽华[2] 李庠[1] 李育强[1] 匡逢春[2] 

机构地区：[1]湖南省棉花科学研究所,湖南常德415101 [2]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《湖南农业科学（下半月）》2014年第2期31-33,共3页

基　　金：湖南省教育厅项目(12C0187);湖南省科技计划项目(2011 NK3078);湖南省自然科学省市联合基金(12JJ8003);棉花生物学国家重点实验室开放课题(CB2013A14);湖南省棉花产业技术体系(2012)

摘　　要：为了研究农杆菌和质粒DNA两种子房注射法对普通棉花品种的转化效率,以8891棉花为材料,分别在三亚和常德两地进行了转化试验。结果表明:与质粒DNA注射相比,三亚地区农杆菌注射的转化效率高1.25个百分点;但农杆菌子房注射的落铃率高,获得的种子相对较少;而在不同的地点试验,常德地区的落铃率显著高于三亚地区;而质粒DNA直接注射的成铃率更高,虽然转化率相对较低,但也能获得有效的转化植株。In order to study the transformation efficiency of two ovary injection methods （with Agrobacterium tumefaciens or with plasmid DNA） to normal cotton variety 8891, the transformation experiments were conducted in Sanya and Changde, respectively. The results showed that the ovary injection with Agrobacterium tumefaciens in Sanya increased the transformation efficiency by 1.25 percentage points, but it had relatively higher boll shedding rate which causing relatively less seeds, moreover, the boll shedding rate in Changde was significantly higher than that in Sanya; ovary injection with plasmid DNA had relatively higher boll setting rate, and it can obtain the effective transformation plant although the transformation efficiency was relatively lower.

关 键 词：农杆菌子房注射 质粒DNA子房注射 棉花 遗传转化 

分 类 号：Q789[生物学—分子生物学]