白术组分拆分及组间差异度评价方法的建立  

A Method for Evaluation of Non-similarity Degrees between Splitting Fractions of Atractylodis Macrocephalae Rhizoma

作　　者：李玲辉[1] 冉小库[1] 徐煜彬[1] 李斌[1] 窦德强[1] 

机构地区：[1]辽宁中医药大学药学院,大连116600

出　　处：《分析化学》2014年第3期343-348,共6页Chinese Journal of Analytical Chemistry

基　　金：国家重点基础研究计划(973计划)项目(No.2013CB531803);2013辽宁省高等学校创新团队课题(No.LT2013020)资助~~

摘　　要：通过多种分离方法联合应用,将白术化学成分拆分成5个部分,各拆分组分组内指纹图谱相似度评价表明,拆分工艺稳定、重现性良好。采用差异度表征中药化学拆分组分间的不交叉程度。利用主成分分析、聚类分析及夹角余弦、平方欧式距离和生药峰面积交叉分析法对白术化学拆分组分进行不交叉的定性与定量分析,表明白术化学拆分组分组间差异度不低于85%。其中生药峰面积差异度分析法是在中药指纹图谱基础上建立的表征中药拆分组分差异度的新方法。通过与不同分析方法比较,发现其较适合中药药效或药性物质研究中的拆分组分间不交叉程度的评价。Five fractions were split for the chemical components of Atractylodis macrocephalae rhizome by the combination application of various separation methods and the over 90% similarity of HPLC fringerprints within each fractions indicated the good repeatability for the splitting procedure. Further, a term of nonsimilarity degree （NSD） was introduced to measure the unoverlapping property of the chemical fractions and different statistic analyses, including principal component analysis, cluster analysis, angle cosin analysis, squared euclidean distance and the NSD of peak areas of crude drug were used to qualitatively and quantitatively analyze their unoverlapping property, revealing the NSD among different fractions was calculated above 85%. Among them, the NSD of peak area of crude drug established on the basis of HPLC fingerprints of crude drug is more suitable for the NSD evaluation of chemical splitting fractions of crude drug comparing with other statistical methods and practical for reflecting the real content-activity relationship in the subsequent exploration of effective substances of crude drugs. This research provides a new effective method to evaluate the chemical difference of splitting fractions of Chinese medicine and lay the foundation for exploring the effective and property-flavor substances or of Atractylodes macrocephala Koidz.

关 键 词：白术 化学组分拆分 组间差异度分析 

分 类 号：R284[医药卫生—中药学]