基于石墨烯和室温离子液体复合物溶胶修饰的玻碳电极制备尿酸电化学传感器  

Preparation of Uric Acid Electrochemical Sensor Based on Graphene/Room Temperature Ionic Liquids Nanocomposite Sol Modified Glassy Carbon Electrode

作　　者：付海莹 王建秀[1] 邓留[1] 

机构地区：[1]中南大学化学化工学院,有色金属资源化学教育部重点实验室,长沙410083

出　　处：《分析化学》2014年第3期441-445,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.21175157);中国博士后基金(No.2011M500126,2012T50656)资助项目~~

摘　　要：将石墨烯（GN）与室温离子液体（IL）1-丁基-3-甲基咪唑六氟磷酸盐（BMIMPF6）,以适当比例研磨成胶状IL-GN,修饰在玻碳电极（GC）上制备了IL-GN/GC.利用原子力显微镜AFM表征IL-GN的形成.由于石墨烯和室温离子液体的协同作用,该电极显示了对H2O2良好的催化性能,基于尿酸氧化酶将之制备成生物传感器,用于尿酸（UA）直接电化学检测,并进行了传感器的抗干扰性能及实际血样中尿酸的检测实验.结果表明,此传感器检测尿酸的线性范围为0.002～ 4.5 mmol/L,相关系数为0.995,检出限为0.85μmol/L,响应时间为10 s.此传感器制备简便,稳定性好,抗干扰能力强,可用于实际血清中尿酸的检测,为尿酸的测定提供了新方法.Graphene/Room temperature ionic liquids （GN/IL） nanocomposite was prepared by grinding graphene and ionic liquid 1-butyl-3-methylimidazolium bromide （BMIMPF6 ） which mixed together in appropriate proportion. Atomic force microscopy （AFM） was utilized to characterize the formation of the GN-ILs. Due to the synergistic effect between ionic liquids and graphene, the nanoeomposite exhibited excellent performance toward H202 reduction. A novel uric acid （UA） electrochemical sensor was fabricated based on uricase-GN/IL modified glassy carbon electrode. The experimental results showed that the response displayed a good linear response toward UA in the concentration range from 0. 002- 4. 5 mmol/L. The correlation coefficient was 0. 995 and the detection limit was 0. 85 μmol/L. The easily prepared electrochemical sensor had favorable stability and selectivity and could be applied to the quick determination of UA in human serum, thus providing a new UA detection method for clinical trial.

关 键 词：石墨烯 室温离子液体 尿酸 电化学传感器 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]