皮下注射与口服地塞米松诱发Balb／c小鼠卡氏肺孢子虫肺炎比较研究  

A comparative study of two Pneumocystis carinii pneumonia models of Balb/c mice induced by dexamethasone through subcutaneous injection and oral route

作　　者：胡晓彤[1] 潘智华[1] 

机构地区：[1]徐州医学院形态学教学实验中心,江苏 徐州221002

出　　处：《徐州医学院学报》2014年第2期99-102,共4页Acta Academiae Medicinae Xuzhou

摘　　要：目的：比较皮下注射和口服地塞米松建立Balb/c小鼠卡氏肺孢子虫肺炎（ PCP）模型的差异，探索2种方法的优劣。方法将雌性Balb/c小鼠随机分为地塞米松皮下注射组（注射组）、地塞米松口服组（口服组）和对照组。注射组采用每3天皮下注射地塞米松1次（0．5 mg/次，共16次）的方法诱发PCP；口服组经口灌服1 mg/L的地塞米松溶液1 ml，持续6周；对照1组注射等体积生理盐水；对照2组不给任何药物。结果注射组无死亡小鼠，51天后卡氏肺孢子虫（PC）总感染率为90％（27/30）；口服组2只小鼠死亡，63天后PC总感染率为67％（20/30）；2组比较差异有统计学意义（P＜0．05）。对照组未检出PC包囊。结论皮下注射和口服地塞米松方法均可成功建立Balb/c小鼠PCP动物模型。皮下注射方法与口服方法比较，具有感染率高、死亡率低、诱导时间短的优点。Objective To compare the difference between two Pneumocystis carinii pneumonia （ PCP ） models of Balb/c mice induced by dexamethasone through subcutaneous injection and oral administration .Methods Female Balb/c mice were randomly divided into a subcutaneous injection of dexamethasone group （Injection）, an oral dexamethasone group （ Oral） and control groups .The Injection group was subcutaneously injected with dexamethasone once every three days （0.5 mg/per time, 16 times in total） to induce PCP.The oral group was orally taken 1 ml of 1 mg/L dexametha-sone for consecutive 6 weeks.The mice in the control group 1 were injected with an equal volume of normal saline , while those in the control group 2 did not receive any medication .Results In the Injection group , no dead mice were reported and fifty-one days later, the total infection rate of Pneumocystis carinii was 90%（27/30）.In the Oral group, two dead mice were found and sixty -three days later the total infection rat was 67% （ 20/30 ） .The differences were statistically significant （P〈0.05）.No Pneumocystis carinii was detected in control groups .Conclusion The PCP model of Balb/c mice can be successfully established through induction of dexamethasone through both subcutaneous injection and oral ad -ministration.Compared with oral route , subcutaneous injection can cause a high infection rate , a low mortality rate and short induction duration .

关 键 词：BALB C小鼠 地塞米松 卡氏肺孢子虫肺炎 动物模型 GIEMSA染色 

分 类 号：R563.1[医药卫生—呼吸系统]