黄芪注射液对免疫功能低下小鼠脾淋巴细胞的影响  

Effects of Astragalus Injection on Spleen Lymphocytes in Immunodeficient Mice

作　　者：王墅塾 王梓楠[1] 赵自明 魏小勇[1] 杜铁良 李琳琳[3] 杨巧红[1] 

机构地区：[1]广州中医药大学,广东广州510405 [2]广东省中医药工程技术研究所,广东广州510095 [3]南方医科大学,广东广州510515

出　　处：《动物医学进展》2014年第4期63-66,共4页Progress In Veterinary Medicine

基　　金：广东省大学生创新创业训练计划项目(1057213008)

摘　　要：观察黄芪注射液(AI)对地塞米松(Dex)所致免疫功能低下小鼠脾淋巴细胞凋亡与增殖的影响,并探讨其作用机制。将72只昆明小鼠随机均分为对照组,模型组,黄芪注射液高、中、低剂量组(2.0、1.0、0.5g/kg),白细胞介素-2(IL-2)组(5IU/kg),除对照组外,其余小鼠均采用Dex(25mg/kg)腹腔注射复制小鼠脾脏淋巴细胞凋亡模型。观察黄芪注射液对小鼠脾脏指数的影响及脾脏的形态学改变,采用免疫组织化学法检测小鼠脾脏淋巴细胞天冬氨酸特异性的半胱氨酸蛋白酶-3(Caspase-3)与增殖细胞核抗原(PCNA)的表达。结果表明,黄芪注射液能明显改善萎缩的脾脏,增加脾脏指数(P<0.05);黄芪各剂量组小鼠脾脏皮质细胞明显增多;同时发现黄芪注射液能降低Caspase-3的表达,并能提高PCNA的表达(P<0.05,P<0.01)。说明黄芪注射液有明显的抗脾脏萎缩及抑制脾脏淋巴细胞凋亡的作用,其作用机制可能与下调Caspase-3及上调PCNA的基因表达相关。To study the effects of astragalus injection（AI） on promoting spleen lymphocyte apoptosis and proliferation in immunodeficient mice by dexamethasone（Dex）, 72 KM mice were randomly divided into six groups： control group, model group, interleukin-2 group（5 IU/kg）, high AI group（2 g/kg）, middle AI group（1 g/kg） and low AI group（0.5 g/kg）. Besides control group,the mice in other five groups were injected Dex 5 days for immunodeficient mouse model. The effects of AI on the spleen index and morphological changes in the spleen were observed,Casepase-3 and PCNA were detectedby immunohistochemistry. AI can significantly improve the atrophy of the spleen, and increase spleen index （P〈0.05）;AI can significantly increase cortical cells of the spleen and splenic lymphocytes;Also AI can reduce the expression of Caspase-3, while improve the expression of PCNA （P〈0.05, P〈0.01）. AI has significant anti-spleen atrophy and inhibition of apoptosis of spleen lymphocytes, and its mechanism may be by down Caspase-3 and up PCNA related-gene expression.

关 键 词：黄芪注射液 免疫功能低下 脾淋巴细胞 凋亡 增殖 小鼠 

分 类 号：S853.7[农业科学—临床兽医学]