泰勒焦虫表面抗原TaSP、spag-1和Tams1基因片段克隆与序列分析  

Cloning and Sequence Analysis of TaSP, Spag-1 and Tams1 Gene Fragments of Theileria Annulata Surface Antigen

作　　者：郭会玲[1] 易忠[1] 艾尼瓦尔.台外库力 陶兴洋 斯马依.伊斯拉木 达力夏.孜乃提 包拉提 王延[1] 薛英[1] 魏玉荣[1] 黄炯[1] 

机构地区：[1]新疆畜牧科学院兽医研究所,新疆乌鲁木齐830000 [2]新疆喀什地区动物疾病控制与诊断中心,新疆喀什844000 [3]新疆克拉玛依绿成农业开发有限责任公司,新疆克拉玛依834000 [4]新疆塔什库尔干县畜牧兽医站,新疆塔什库尔干845250 [5]新疆塔什库尔干县达布达尔乡畜牧兽医站,新疆塔什库尔干845250 [6]新疆昭苏县畜牧兽医站,新疆昭苏835600

出　　处：《草食家畜》2014年第2期55-59,共5页Grass-Feeding Livestock

基　　金：国家自然科学基金项目(31160505);十二五"国家科技支撑计划(2013BAD2B04)

摘　　要：为了解泰勒焦虫表面抗原基因特性,利用PCR方法对实验室保存的泰勒焦虫细胞表面抗原spag-1、Tams1和TaS部分基因片段进行克隆与序列分析。PCR结果显示所扩增的spag-1、Tams1和TaSP部分基因长度分别为372bp,324bp和321bp。系统发育树分析表明,与本次测序表面抗原TaSP和Tams1基因片段同源性最高的虫株登录号分别为FJ895154和AF214797的泰勒焦虫株,同源性分别高达100%与99.69%;与spag-1基因片段同源性最高的虫株登录号为X78188、XM949884和M63017,它们之间同源性为100%。To study the genes characteristics of Theileria annulata surface antigen, gene fragments of spag-1 and Tamsl and Tasp were cloned by PCR and sequenced. The result showed that PCR products of genes ffagme nt of TaSP,spag-1 and Tams1 were 372bp,324bp and 321bp, respectively.After sequenced, phylogenetic tree was drew using genes fragment TaSP, Tamsl and spag-l.The analysis of Phylogenetic tree revealed that he highest homology is 100% with the stain of accession number FJ895154 based on Tasp gene sequences of Theileria annulata, and it has a high homology with the stain of accession number AF214797 of 99.69% based on Tasp gene sequences of Theileria annulata. The sequence of spag-1 genes fragment is most similar to the stain of accession number X78188,XM949884 and M63017 with 100%,respectively.

关 键 词：泰勒焦虫 TaSP spag-1 Tams1 克隆 序列分析 

分 类 号：S855.9[农业科学—临床兽医学]