正加速度暴露后大鼠心室肌LC3发生高表达变化  

Over-expression of LC3 in ventricular muscle of rats after exposure to +Gz

作　　者：赵疆东[1] 王永春[1] 石菲[1] 詹皓[2] 孙喜庆[1] 

机构地区：[1]第四军医大学航空航天医学系航空航天生物动力学教研室,陕西西安710032 [2]空军航空医学研究所,北京100142

出　　处：《解放军医学院学报》2014年第4期357-359,共3页Academic Journal of Chinese PLA Medical School

基　　金：国家自然科学基金项目(81073083)~~

摘　　要：目的探讨正加速度(+Gz)暴露后大鼠心室肌自噬相关基因微管相关蛋白轻链3(LC3)的表达变化。方法雄性SD大鼠18只,随机分为对照组和+10 Gz/5 min暴露组。分别于+Gz暴露后6 h、24 h取心室肌组织,采用RT-PCR和Western blot方法测定LC3 mRNA及蛋白表达的变化。结果与对照组相比,+Gz暴露后6 h心室肌LC3 mRNA及蛋白表达均显著增强(P<0.05),暴露后24 h其表达量仍显著增加(P<0.05)。结论 +10 Gz/5 min暴露可诱导大鼠心室肌自噬相关基因LC3的高表达,提示高+Gz可能引起了心室肌自噬的激活。Objective To study the expression of autophagy-related marker LC3 in ventricular muscle of rats after exposure to ＋Gz. Methods Eighteen male SD rats were randomly divided into control group and ＋10 Gz/5 min group. Their ventricular muscle tissue samples were taken at 6 and 24 h after exposure to ＋10 Gz. Expressions of LC3 mRNA and protein were detected by RT-PCR and Western blot, respectively. Results The expression levels of LC3 mRNA and protein were significantly higher in ＋10 Gz/5 min group than in control group at 6 and 24 h after exposure to ＋Gz （P 〈 0.05）. Conclusion Exposure to ＋10 Gz can induce over- expression of LC3 in ventricular muscle of rats by activating the autophagocytic function of their ventricular muscle.

关 键 词：正加速度 自噬 微管相关蛋白轻链3 大鼠 心室肌 

分 类 号：R852.21[医药卫生—航空、航天与航海医学]