P-选择素基因多态性与冠心病的相关性  

Correlation between P-selectin gene polymorphism and coronary heart disease

作　　者：霍小蓓 张蕴莉[1] 刘彦彦[1] 

机构地区：[1]辽宁医学院附属第一医院检验科,辽宁锦州121001

出　　处：《解放军医学院学报》2014年第4期374-378,共5页Academic Journal of Chinese PLA Medical School

摘　　要：目的研究P-选择素基因多态性与冠心病(coronary heart disease,CHD)易感性之间的相关性。方法以2012年6月-2013年4月辽宁医学院附属第一医院心内科病房的200例冠心病患者为病例组,210例健康体检者为对照组,应用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法和DNA测序法,检测两组人群P-选择素基因启动子区-2123C/G、-1969G/A和-1817T/C的基因多态性,并将冠心病组分为心绞痛、心肌梗死和缺血性心力衰竭组,研究不同类型冠心病组与对照组之间P-选择素基因启动子区-2123C/G、-1969G/A和-1817T/C位点基因多态性分布的差异。结果 P-选择素基因-2123C/G位点基因型和等位基因频率在冠心病组和对照组间的分布差异有统计学意义(χ2=6.124,P=0.047;χ2=7.355,P=0.008),并且在心肌梗死组与对照组、缺血性心力衰竭组与对照组的分布差异也具有统计学意义(χ2=6.473,P=0.039;χ2=13.944,P=0.001)。-1817T/C和-1969G/A位点基因型和等位基因频率在冠心病组和对照组间以及不同类型冠心病组和对照组间的分布差异均无统计学意义(P>0.05)。结论 P-选择素基因-2123C/G位点多态性与冠心病易感性之间具有相关性。Objective To study the correlation between P-selectin gene polymorphism and coronary heart disease（CHD）. Methods Two hundred CHD patients admitted to our hospital from June 2012 to April 2013 served as a CHD group and 210 subjects undergoing physical examination served as a control group in this study. Polymorphism of their P-selectin genes -2123C/G,-1969G/A and -1817T/C was detected by PCR-RELP and DNA sequencing, respectively. CHD patients were further divided into angina pectoris group, myocardial infarction group and ischemic heart failure group. Difference in polymorphism of P-selectin genes -2123C/G, -1969G/A and -1817T/C was observed in different types of CHD. Results The frequency of P-selectin gene -2123C/G and allelic gene was significantly higher in CHD group than in control group（x 2=6.124, P=0.047; X 2=7.355, P=0.008） and in myocardial infarction group and ischemic heart failure group than in control group（x 2=6.473, P=-0.039; X 2=13-944, P=0.001）. However, no significant difference was found in the frequency of P-selectin genes -1817T/C and -1969G/A and allelic gene between CHD group and control group（P 〉 0.05）. Conclusion P-selectin gene -2123C/G polymorphism is correlated with CHD.

关 键 词：P-选择素 冠心病 基因多态性 基因型 单倍型 

分 类 号：R541.4[医药卫生—心血管疾病]